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Microsatellite instability in Russian patients with gastric cancer
https://doi.org/10.25557/2073-7998.2024.03.31-37
Abstract
Introduction. Gastric cancer (GC) is a common malignant disease that has a significant impact on patients’ quality of life and mortality rate. Microsatellite instability (MSI) is a genetic change that can influence cancer progression and prognosis. This study examines the frequency of MSI in GC in Russian patients, as well as analyzes its prognostic significance.
Aim: to determine the frequency of MSI in patients with gastric cancer in Russia and its relationship with clinical parameters and determine the prognostic significance.
Methods. The study material included 160 paired samples of tumor and non-tumor tissue from patients undergoing surgery for gastric cancer. Microsatellite instability was determined by DNA fragment analysis using five mononucleotide markers (NR21, NR24, NR27, BAT25 and BAT26). Samples were compared using Fisher’s exact test. For comparisons of more than three groups, the chi-square test was used. Overall probability of survival (OS) was calculated by the Kaplan-Meier product limit method from the date of surgery to death from any cause and compared statistically using the Mantel-Haenszel test.
Results. According to the study results, MSI was found in 28 out of 160 patients, representing 17.5%. When analyzing the associations of MSI with clinical and pathological characteristics of patients, a significant association was revealed with age over 50 years (p=0.038), tumor size (T3-T4) (p=0.017), metastases to regional lymph nodes (N1-N3) (p= 0.028), distant metastases (M1) (p=0.009) and intestinal type according to Lauren’s classification (p=0.046).
Supporting agencies: The work was carried out with financial support from the Russian Science Foundation (grant No. 20-75-10117-P).
About the Authors
A. D. Molchanov
I.M. Sechenov First Moscow state medical university;
N.N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
8-2, Trubetskaya st., Moscow, 119991;
23, Kashirskoe shosse, Moscow, 115478
A. S. Andrianov
I.M. Sechenov First Moscow state medical university
Russian Federation
Russian Federation
8-2, Trubetskaya st., Moscow, 119991
A. S. Vasilyeva
N.N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
23, Kashirskoe shosse, Moscow, 115478
I. V. Bure
I.M. Sechenov First Moscow state medical university
Russian Federation
Russian Federation
8-2, Trubetskaya st., Moscow, 119991
E. B. Kuznetsova
I.M. Sechenov First Moscow state medical university;
Research Centre for Medical Genetics
Russian Federation
Russian Federation
8-2, Trubetskaya st., Moscow, 119991;
1, Moskvorechye st., Moscow, 115522
E. A. Alekseeva
I.M. Sechenov First Moscow state medical university;
Research Centre for Medical Genetics
Russian Federation
Russian Federation
8-2, Trubetskaya st., Moscow, 119991;
1, Moskvorechye st., Moscow, 115522
M. V. Nemtsova
I.M. Sechenov First Moscow state medical university;
Research Centre for Medical Genetics
Russian Federation
Russian Federation
8-2, Trubetskaya st., Moscow, 119991;
1, Moskvorechye st., Moscow, 115522
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Review
For citations:
Molchanov A.D., Andrianov A.S., Vasilyeva A.S., Bure I.V., Kuznetsova E.B., Alekseeva E.A., Nemtsova M.V. Microsatellite instability in Russian patients with gastric cancer. Medical Genetics. 2024;23(3):31-37. (In Russ.) https://doi.org/10.25557/2073-7998.2024.03.31-37
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