Copy number of ribosomal genes in woman's genome is associated with IVF outcome and pregnancy complications
https://doi.org/10.25557/2073-7998.2019.11.14-25
Abstract
As pregnancy is a stressful load for a woman, any stress-resistance factor is relevant to it. According to recent reports, ribosomal gene copy number in the genome is associated with the individual stress-resistance. We determined copy numbers of ribosomal DNA (rDNA) in genomes of pregnant women with normal and complicated pregnancy, and women after in vitro fertilization (IVF) procedure. We also measured the contents of GC-rich rDNA in cell-free DNA (cfDNA) derived from normal controls and complicated pregnancy cases. We have shown that genomes of more than a half of DNA samples from women with pregnancy pathology harbor either more, or less rDNA copies than any woman from the control group. We also found higher rDNA contents in cfDNA isolated from complicated pregnancy cases suggesting the presence of a permanent cell death process in pathology cases. A principal conclusion can be made: women with low rDNA copy numbers and with very high numbers can have higher cell death rates and belong to the risk group. The parameter «rDNA copy number in woman’s genome» can be an additional prognostic marker for eventual pregnancy complications in the woman. The numbers of rDNA copies in the genomes of women with failed IVF attempts was significantly lower than in the genomes of patients with succesfull outcome, suggesting that rDNA copy number in the genome is one of the factors that affect the success of the IVF procedure. If the individual rDNA copy number is under 330, the risk of IVF failure is high. Further studies are warranted.
About the Authors
L. N. Porokhovnik
Research Centre for Medical Genetics n.a. N.P. Bochkov
Russian Federation
Russian Federation
N. N. Veiko
Research Centre for Medical Genetics n.a. N.P. Bochkov
Russian Federation
Russian Federation
E. S. Ershova
Research Centre for Medical Genetics n.a. N.P. Bochkov; V.A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology
Russian Federation
Russian Federation
A. A. Poletkina
Research Centre for Medical Genetics n.a. N.P. Bochkov
Russian Federation
Russian Federation
G. V. Shmarina
Research Centre for Medical Genetics n.a. N.P. Bochkov
Russian Federation
Russian Federation
O. A. Dolgikh
Research Centre for Medical Genetics n.a. N.P. Bochkov
Russian Federation
Russian Federation
P. A. Klimenko
N.I. Pirogov Russian National Research Medical University
Russian Federation
Russian Federation
M. P. Klimenko
N.I. Pirogov Russian National Research Medical University
Russian Federation
Russian Federation
K. G. Avetisova
N.I. Pirogov Russian National Research Medical University
Russian Federation
Russian Federation
E. V. Kostyuk
N.I. Pirogov Russian National Research Medical University
Russian Federation
Russian Federation
M. A. Kurtser
N.I. Pirogov Russian National Research Medical University
Russian Federation
Russian Federation
V. M. Pisarev
V.A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology
Russian Federation
Russian Federation
V. L. Izhevskaya
Research Centre for Medical Genetics n.a. N.P. Bochkov
Russian Federation
Russian Federation
S. I. Kutsev
Research Centre for Medical Genetics n.a. N.P. Bochkov
Russian Federation
Russian Federation
S. V. Kostyuk
Research Centre for Medical Genetics n.a. N.P. Bochkov; V.A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology
Russian Federation
Russian Federation
Review
For citations:
Porokhovnik L.N., Veiko N.N., Ershova E.S., Poletkina A.A., Shmarina G.V., Dolgikh O.A., Klimenko P.A., Klimenko M.P., Avetisova K.G., Kostyuk E.V., Kurtser M.A., Pisarev V.M., Izhevskaya V.L., Kutsev S.I., Kostyuk S.V. Copy number of ribosomal genes in woman's genome is associated with IVF outcome and pregnancy complications. Medical Genetics. 2019;18(11):14-25. (In Russ.) https://doi.org/10.25557/2073-7998.2019.11.14-25
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