EXPRESSION OF CIRCADIAN GENES CLOCK, BMAL1 AND PER1 IN BUCCAL CELLS OF DONORS WITH ESSENTIAL HYPERTENSION
https://doi.org/10.1234/XXXX-XXXX-2013-4-27-31
Abstract
The analysis of differences in the expression rates of the main circadian genes CLOCK, BMAL1, and PER1 in human oral mucosa cells between patients with essential hypertension (EH) and healthy individuals was carried out for the first time. The expression levels of the genes were calculated at 9:00 a.m., 1:00 p.m., and 5:00 p.m. Patients with EH exhibited lower expression levels of the CLOCK gene at 1:00 p.m. and 5:00 p.m. as compared with control group. The expression levels of the BMAL1 gene were significantly lower at 9:00 a.m. and 1:00 p.m. in comparison with healthy individuals. Patients with EH exhibited lower expression levels of the PER1 gene at all time points as compared to control group. The signifi cant positive corre i ation between expression levels of CLOCK and PER1, BMAL1 and PER1 genes was shown in control group and donors with EH at all time points using Spearman rank correlation analysis.
About the Authors
I. V. Kurbatova
Institute of Biology Karelian Research Center Russian Academy of Sciences
Russian Federation
Russian Federation
L. V. Topchieva
Institute of Biology Karelian Research Center Russian Academy of Sciences
Russian Federation
Russian Federation
V. A. Korneva
Faculty therapy department of Petrozavodsk State University
Russian Federation
Russian Federation
S. N. Kolomeichuk
Institute of Biology Karelian Research Center Russian Academy of Sciences
Russian Federation
Russian Federation
N. N. Nemova
Faculty therapy department of Petrozavodsk State University
Russian Federation
Russian Federation
References
1. Курбатова И.В., Коломейчук С. Н., Топчиева Л.В., Корнева В.А., Немова Н.Н. Экспрессия генов циркадных ритмов CLOCK, BMAL1 и PER1 в клетках буккального эпителия человека в зависимости от полиморфных вариантов гена CLOCK// Доклады академии наук. — 2012. — Т. 446, №6. — С. 703—706.
2. Курбатова И.В., Топчиева Л.В., Коломейчук С.Н., Корнева В.А., Немова Н.Н. Полиморфные маркеры гена транскрипционного фактора CLOCK и риск возникновения эссенциальной артериальной гипертензии и ишемической болезни сердца у жителей Республики Карелия // Актуальные проблемы лабораторной диагностики и биотехнологии: Всерос. на-уч.-практ. конф.: Сб. тез. — Кемерово, 2012. — С. 51—52.
3. Bjarnason G.A., Jordan R.C., Wood P.A., Li Q., Lincoln D.W., Sothern R.B., Hrushesky W.J., Ben-David Y. Circadian expression of clock genes in human oral mucosa and skin: association with specific cell-cycre phares // Am. J. Pathol. — 2001. — Vol. 158, №5. — P. 1793—1801.
4. Chen S.T., Choo K.B., Hou M.F., Yeh K.T., Kuo S.J., Chang J.G. Deregurated expression of the PER1, PER2 and PER3 genes in breast cancers // Carcinogenesis. — 2005. — Vol. 26, №7. — P. 1241 — 1246.
5. Chu L.W., Zhu Y., Yu K., Zheng T., Yu H., Zhang Y., Sester-henn I., Chokkahngam A.P., Danrorth K.N., Shen M.C., Stanc-zyk F.Z., Gao Y.T., Hsing A.W. Variants in circadian genes and prostate cancer risk: a population-based study in China // Prostate Cancer Prostatic Dis. — 2008. — Vol. 11, №4. — P. 342—348.
6. Doi M., Hirayama J., Sassone-Corsi P. Circadian regulator CLOCK is a histone acetyltransferase // Cell. — 2006. — Vol. 125, №3. — P. 497—508.
7. Englund A., Kovanen L., Saarikoski S.T., Haukka J., Reuna-nen A., Aromaa A., Lonnqvist J., Partonen T. NPAS2 and PER2 are linked to risk factors of the metabolic syndrome // J. Circadian Rhythms. — 2009. — Vol. 7, №5. — P. 1—9.
8. Gomez-Abelian P., Madrid J.A., Luj an J.A., Frutos M.D., Gonzаlez R., Martinez-Augustin O., de Medina F.S., Ordovas J.M., Garaulet M. Sexual dimorphism in clock genes expression in human adipose tissue // Obes. Surg. — 2012. — Vol. 22, №1. — P. 105—112.
9. Guney H.Z., Uluoglu C., Hodoglugil U., Gorgun C.Z., Yamanoglu T.M., Abacioglu N., Zengil H. Biological-time-dependent differences in effect of verapamil on rat aorta and influence of endothehum // Chronobiol. Int. — 1999. — Vol. 16, №6. — P. 779—787.
10. Honma S., Ikeda M., Abe H., Tanahashi Y., Namihira M., Honma K., Nomura M. Circadian oscillation of BMAL1, a partner of a mammalian clock gene Clock, in rat suprachiasmatic nucleus // Bi-ochem. Biophys. Res. Commun. — 1998. — Vol. 250, №1. — P. 83—87.
11. KruglugerW., Brandstaetter A., Kallay E., SchuellerJ., Krex-ner E., Kriwanek S., Bonner E., Cross H.S. Regulation of genes of the circadian clock in human colon cancer: reduced period 1 and dihydropyrimidine dehydrogenase transcription correlates in high-grade tumors // Cancer Res. — 2007. — Vol. 67, №16. — P. 7917—7922.
12. Maemura K., Takeda N., Nagai R. Circadian rhythms in the CNS and peripheral clock disorders: role of the biological clock in cardiovascular diseases // J. Pharmacol. Sci. — 2007. — Vol. 103, №2. — P. 134—138.
13. Miyazaki M., Schroder E., Edelmann S.E., Hughes M.E., Kornacker K., Balke C.W., Esser K.A. Age-associated disruption of molecular clock expression in skeletal muscle of the spontaneously hypertensive rat // PLoS One. — 2011. — Vol. 6, №11, e27168. — P. 1—11.
14. Mrosovsky N., Edelstein K., Hastings M.H., Maywood E.S. Cycle of period gene expression in a diurnal mammal (Spermophilus
15. tridecemlineatus): implications for nonphotic phase shifting // J. Biol. Rhythms. — 2001. — Vol. 16, №5. — P. 471—478.
16. Reilly D.F., Westgate E.J., FitzGerald G.A. Peripheral circadian clocks in the vasculature // Arterioscler. Thromb. Vasc. Biol. — 2007. — Vol. 27, №8. — P. 1694—1705.
17. Rudic R.D., McNamara P., ReiUy D., Grosrer T., Curr tis A.M., Price T.S., Panda S., Hogenesch J.B., FitzGerald G.A. Bioinformatic analysis of circadian gene oscillation in mouse aorta // Circulation. — 2005. — Vol. 112, №17. — P. 2716—2724.
18. Sarur R.M, Emoto N., Nonaka H., Okura R., Nishimu-ra M., Yagita K., van der Horst G.T., Matruo M., Okamura H., Yokoyama M. Circadian clock genes directly regulate expression of the Na(+)/H(+) exchanger NHE3 in the kidney // Kidney Int. — 2005. — Vol. 67, №4. — P. 1410—1419.
19. Sato T.K., Panda S., Miraglia L.J., Reyes T.M., Rudic R.D., McNamara P., Naik K.A., FitzGerald G.A., Kay S.A., Hogenesch J.B. A functional genomics strategy reveals Rora as a component of the mammahrn cirradian clock // Neuron. — 2004. — Vol. 43, №4. — P. 527—537.
20. Scott E.M., Carter A.M., Grant P.J. Association between polymorphisms in the Clock gene, obesity and the metabolic syndrome in man // Int. J. Obes. (Lond.). — 2008. — Vol. 32, №4. — P. 658—662.
21. Stamenkovic JA, Olsson A.H., Nagorny C.L., Malmgren S., Dekker-Nitert M., Ling C., Mulder H. Reguration of core clock genes in human isrets // MeraboUsm. — 2012. — Vol. 61, №7. — P. 978—985.
22. Takahashi J.S., Hong H.K., Ko C.H., McDearmon E.L. The genetics of mammalian circadian order and disorder: implications for physiology and disease // Nat. Rev. Genet. — 2008. — Vol. 9, №10. — P. 764—775.
23. Von Schantz M. Phenotypic effects of genetic variability in human clock genes on circadian and sleep parameters // J. Genet. — 2008. — Vol. 87, №5. — P. 513—519.
24. Winter S.L., Bosnoyan-Collins L., Pinnaduwage D., Andru-lis I.L. Expression of the Circadian Clock Genes Per1 and Per2 in Sporadic and Familial Breast Tumors // Neoplasia. — 2007. — Vol. 9, №10. — P. 797—800.
25. Woon P.Y., Karsaki P.J., Braganca J., Bihoreau M.T., Levy J.C., Farrall M., Gauguier D. Aryl hydrocarbon receptor nuclear translocator-like (BMAL1) is associated with susceptibility to hypertension and type 2 diabetes // Proc. Natl. Acad. Sci. USA. — 2007. — Vol. 104, №36. — P. 14412—14417.
26. Young M.E., Razeghi P., Taegtmeyer H. Clock genes in the heart: characterization and attenuation with hypertrophy // Circ. Res. — 2001. — Vol. 88, №11. — P. 1142—1150.
Review
For citations:
Kurbatova I.V., Topchieva L.V., Korneva V.A., Kolomeichuk S.N., Nemova N.N. EXPRESSION OF CIRCADIAN GENES CLOCK, BMAL1 AND PER1 IN BUCCAL CELLS OF DONORS WITH ESSENTIAL HYPERTENSION. Medical Genetics. 2013;12(4):27-31. (In Russ.) https://doi.org/10.1234/XXXX-XXXX-2013-4-27-31
Views:
939
Email this article 