Methylation index of imprinted genes GRB10 and GNAS in abnormal human embryo development

Present study analyzes the methylation index of the imprinted genes GNAS ( NESP55 ) and GRB10 in the group of first trimester spontaneous abortions with normal karyotype. The DNA samples derived from extraembryonic mesoderm of 47 spontaneous abortions and 45 induced abortions were examined. A significant increase in the methylation index of the imprinted gene NESP55 and a decrease in GRB10 in the spontaneous abortions were observed. Based on the function of this imprinted genes, it can be assumed that an increase in the methylation index of the NESP55 gene and its decrease in GRB10 could enhance the suppression of embryo growth and lead to a possible disturbance of embryo development.

геномный импринтинг, GRB10, NESP55, индекс метилирования, невынашивание беременности, спонтанные абортусы, эпимутации, genomic imprinting, GRB10, NESP55, methylation index, pregnancy loss, spontaneous abortion, epimutations

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