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miR-23b-3p and miR-27b-3p demonstrate a strong inverse correlation of DNA expression and methylation in the MIR23B promoter region in carotid atherosclerotic plaques
https://doi.org/10.25557/2073-7998.2026.02.59-62
Abstract
A change in microRNA expression, which can be influenced by a change in the methylation level of microRNA gene (MIR) promoters, is observed as a biochemical marker of carotid atherosclerotic plaques (ASP) instability. The aim of our work was to identify microRNAs with the strongest correlation between the level of methylation of CpG sites in the promoter region of MIR genes and the expression of mature microRNAs in atherosclerotic plaques of human carotid arteries. DNA and microRNA for analysis were isolated from the ASP of patients with advanced carotid atherosclerosis (n=14). DNA methylation was analyzed by the methyloarrays, and microRNA expression was analyzed using the microRNA–seq. The expression of miR-23b-3p and miR-27b-3p was inversely correlated with the methylation of cg09951047, cg20355301 and cg13972491. The results obtained indicate the important role of methylation of the MIR23B gene promoter in regulating the expression of the miR-23b/-27b/ -24-1 cluster in atherosclerotic lesions of the carotid arteries.
Supporting agencies: The work was carried out according to the State Assignment of the Ministry of Science and Higher Education No. 122020300041-7
About the Authors
Iu. A. Koroleva
Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Sciences
Russian Federation
Russian Federation
10, Nab. Ushaiki, Tomsk, 634050, Russian Federation
A. A. Zarubin
Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Sciences
Russian Federation
Russian Federation
10, Nab. Ushaiki, Tomsk, 634050, Russian Federation
M. S. Nazarenko
Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Sciences
Russian Federation
Russian Federation
10, Nab. Ushaiki, Tomsk, 634050, Russian Federation
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Review
For citations:
Koroleva I.A., Zarubin A.A., Nazarenko M.S. miR-23b-3p and miR-27b-3p demonstrate a strong inverse correlation of DNA expression and methylation in the MIR23B promoter region in carotid atherosclerotic plaques. Medical Genetics. 2026;25(2):59-62. (In Russ.) https://doi.org/10.25557/2073-7998.2026.02.59-62
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