Studying of association of MnSOD, GPx and TP53 genes polymorphism with frequencies of chromosomal aberrations in human lymphocytes under long-term radon exposure

Relevance. Ionizing radon radiation besides the direct effect, also leads to indirect DNA damage caused by oxidative stress as a result of the action of water radiolysis products. It can be assumed the important role of antioxidant response genes in the development of DNA damage, including such recognized biomarkers of genotoxic and carcinogenic effects as chromosomal aberrations. Goal. To study association of polymorphic variants of 3 protective genes: MnSOD, GPx, Tp53 with the frequency of chromosomal aberrations in blood lymphocytes of adolescents who live for a long time under exposure by excessive level of high-LET radon radiation. Materials and methods. Three single-nucleotide variations in genes were genotyped: MnSOD 47C> T (rs4880), GPx 599 C> T (rs1050450), Tp53 215 G> C (rs1042522), in a group of people exposed for extended periods of exposure to radon in elevated concentrations (> 200 Bq/m^{3). Genotyping was carried out using the real-time PCR method using the technology of competing TaqMan probes. The frequency of chromosome aberrations was calculated by light microscopy. Results. ROC curves analysis obtained by results of genotyping of GPx and Tp53 genes, made it possible to identify this genes as possible «satisfactory» predictors associated with an increase in the proportion of aberrant metaphases: the hereditary variant GPx T, rs1050450 (AUC = 0.603, p = 0.04) in the recessive model, and variant Tp53 C, rs1042522 (AUC = 0.634, p = 0.03) also in the recessive model.} Conclusions. Single-nucleotide variations in genes GPx T, rs1050450 and Tp53 C, rs1042522 may be associated with an increased risk of DNA damage in conditions of prolonged radiation low-dose exposure.

радон, ионизирующее излучение, генетический полиморфизм, антиоксидантные ферменты, глутатионпероксидаза, супероксиддисмутаза, транскрипционный фактор р53, хромосомные аберрации, radon, radiation ionizing, polymorphism genetic, antioxidants, glutathione peroxidase, superoxide dismutase, tumor suppressor protein p53, chromosome aberrations

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