Contribution polymorphism (rs55754655) aldehyde oxidase gene in the development of acute pancreatitis

Abstract

Acute pancreatitis (AP) is a multifactorial disease, the development of which is determined by a complex interaction of multiple genes and various environmental factors, questions relating to genetic mechanisms of AP and its complications have been studied are not enough. An important role in the development of acute pancreatitis is given the action of toxic substances and disruption of the regulation of pro- and antioxidant protection. One of prooxidant enzymes is aldehyde oxidase (AOX1), which plays a substantial role in the metabolism of xenobiotics, drugs and ethanol degradation products. Aldehyde oxidase gene was not considered as a possible candidate susceptibility to acute pancreatitis, although the pathogenetic significance of AOX1 gene against acute pancreatitis is obvious. The aim of this study was to investigate the relationship between polymorphism (rs55754655) AOX1 gene and risk of acute pancreatitis in Russian population. Whole blood samples were obtained from 311 AP patients and 238 healthy controls. Genotyping of polymorphisms (rs55754655) AOX1 gene was performed through a TaqMan assay. Genotype А/G (OR = 1.83 95% CI 1.06-3.17 p = 0.03) was significantly associated with the risk of AP. Stratified analysis showed that the genotype A / G was associated with development of acute pancreatitis in women (OR = 3.36 95% CI 1.25-9.01 p = 0.01). Statistically significant differences in the frequencies of alleles and genotypes of polymorphism in groups of smokers and non-smokers are not installed. It was found that the amount and frequency of administration of alcohol did not change the overall picture of genotype associations with the risk of acute pancreatitis. However, patients with genotype A/G with a history of alcohol drinking 10 or more years have the highest risk to the developing pancreatitis (OR = 2.63 95% CI 1.21-5.7, p = 0.01).