Effects of GSTP1 and GPX4 genetic variants and their gene-gene interaction on the severity of COVID-19
https://doi.org/10.25557/2073-7998.2024.04.45-53
Abstract
Background. Coronavirus disease 2019 (COVID-19) is a highly contagious disease, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Several risk factors of COVID-19 susceptibility and severity are associated with oxidative stress.
Aim: to investigate the association of single nucleotide polymorphisms (SNPs) of glutathione S-transferase pi-1 (GSTP1) and glutathione peroxidase 4 (GPX4) genes with the severity of COVID-19.
Methods. Study subjects were divided into two groups based on the severity of their symptoms. Allele-specific real time polymerase chain reaction (RT-PCR) was used for genotyping, and multifactor dimensionality reduction (MDR) analysis was performed to investigate the SNP-SNP interaction models.
Results. The results showed a significant association of GPX4 rs713041 with the severity of COVID-19 (p=0.035). Most of GPX4 718TT carriers had a severe course of COVID-19 (OR=3.50; 95% CI [1.18-10.35]). The resulted three-locus SNP-SNP interaction model was statistically significant (p=0.0084, OR = 2.42; 95% CI [1.24 - 4.73]). Linkage disequilibrium analysis of GSTP1 SNPs rs1695 and rs1138272 showed a high possibility of linkage disequilibrium between the two sites (D’ = 0.949).
Conclusions: To our knowledge, this is the first study to investigate the association of GSTP1 rs1695, GSTP1 rs1138272, and GPX4 rs713041 and SNP-SNP interaction with the severity of COVID-19 symptoms. The obtained results may serve as a novel potential factor of COVID-19 prognosis.
Supporting agencies: This study was funded by the Ministry of Science and Higher Education of the Russian Federation № FENW-2023-0018.
About the Authors
M. A. Eid
Southern Federal University
Russian Federation
Russian Federation
105/42, Bolshaya Sadovaya st., Rostov-on-Don, 344006
A. A. Aleksandrova
Southern Federal University;
Medical Center “Nauka”
Russian Federation
Russian Federation
105/42, Bolshaya Sadovaya st., Rostov-on-Don, 344006;
23A, Zagorskaya st., Rostov-on-Don, 344034
S. A. Zatonsky
Medical Center “Nauka”;
Rostov State Medical University
Russian Federation
Russian Federation
23A, Zagorskaya st., Rostov-on-Don, 344034;
119, Suvorova st., Rostov-on-Don, 344022
L. V. Gutnikova
Southern Federal University
Russian Federation
Russian Federation
105/42, Bolshaya Sadovaya st., Rostov-on-Don, 344006
T. P. Shkurat
Southern Federal University;
Medical Center “Nauka”
Russian Federation
Russian Federation
105/42, Bolshaya Sadovaya st., Rostov-on-Don, 344006;
23A, Zagorskaya st., Rostov-on-Don, 344034
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For citations:
Eid M.A., Aleksandrova A.A., Zatonsky S.A., Gutnikova L.V., Shkurat T.P. Effects of GSTP1 and GPX4 genetic variants and their gene-gene interaction on the severity of COVID-19. Medical Genetics. 2024;23(4):45-53. https://doi.org/10.25557/2073-7998.2024.04.45-53
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