Влияние генетических вариантов GSTP1 и GPX4 и их межгенного взаимодействия на тяжесть течения COVID-19

Введение. Коронавирусное заболевание 2019 (COVID-19) – высококонтагиозное заболевание, вызываемое коронавирусом SARSCoV-2. Несколько факторов риска восприимчивости и тяжести COVID-19 связаны с окислительным стрессом. Целью настоящего исследования было изучение ассоциации однонуклеотидных полиморфизмов (SNP) генов глутатион S-трансферазы pi-1 (GSTP1) и глутатионпероксидазы 4 (GPx4) с тяжестью заболевания COVID-19.
Методы. Пациенты были разделены на две группы в зависимости от тяжести симптомов. Для генотипирования использовалась аллель-специфическая полимеразная цепная реакция в реальном времени (RT-PCR). Для исследования межгенных взаимодействий проводился MDR-анализ (multifactor dimensionality reduction).
Результаты. Показана значимая ассоциация GPX4 rs713041 с тяжестью течения COVID-19 (p=0,035). У большинства носителей генотипа GPX4 718 (TT) было тяжелое течение COVID-19 (ОШ=3,50; 95% ДИ [1,18-10,35]). Трехлокусная модель взаимодействия SNP-SNP была статистически значимой (p = 0,0084, ОШ = 2,42; 95% ДИ [1,24–4,73]). Анализ неравновесия по сцеплению GSTP1 rs1695 и rs1138272 показал высокую вероятность неравновесия по сцеплению между двумя сайтами (D’ = 0,949).
Выводы. Насколько нам известно, это первое исследование ассоциаций и межгенных взаимодействий GSTP1 rs1695, GSTP1 rs1138272 и GPX4 rs713041 с тяжестью симптомов COVID-19. Полученные результаты могут служить новым потенциальным фактором прогноза COVID-19.

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