Single cell and whole genome amplification product validation of preimplantation genetic testing systems for monogenic disorders
https://doi.org/10.25557/2073-7998.2020.11.63-64
Abstract
Pre-examination single cell validation was performed for preimplantation genetic testing (PGT-M) for 9 monogenic disorders by nested PCR for STR and pathogenic variants. Totally 109 single cells and 24 WGA products (by MDA) were analyzed.
About the Authors
E. V. Soloveva
Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Sciences
Russian Federation
Russian Federation
O. R. Kanbekova
Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Sciences
Russian Federation
Russian Federation
D. I. Zhigalina
Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Sciences
Russian Federation
Russian Federation
N. A. Skryabin
Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Sciences
Russian Federation
Russian Federation
L. I. Minaycheva
Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Sciences
Russian Federation
Russian Federation
References
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Review
For citations:
Soloveva E.V., Kanbekova O.R., Zhigalina D.I., Skryabin N.A., Minaycheva L.I. Single cell and whole genome amplification product validation of preimplantation genetic testing systems for monogenic disorders. Medical Genetics. 2020;19(11):63-64. (In Russ.) https://doi.org/10.25557/2073-7998.2020.11.63-64
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