Next-Generation Sequencing for somatic mutation detection in colorectal cancer and lung cancer

Abstract

Study objective is to assess the prevalence of rare mutations in the EGFR, KRAS, NRAS, BRAF genes, as well as the proportion of the mutant allele frequency (AF) in the samples of patients with lung cancer and colorectal cancer. Materials and Methods: 199 samples of DNA isolated from paraffin blocks was studied using next generation sequencing. Study Results: rare mutations in colorectal cancer were detected in 12.8% of patients. 20% of the samples had AF <15%. Rare mutations in lung cancer were observed in 24.2%. 27% of the samples had AF <15%. Conclusion: Next Generation Sequencing (NGS) may be recommended as a routine method for detecting somatic mutations in a tumor. NGS has great diagnostic capabilities compared to PCR or Sanger sequencing.