Methylation of PNPLA2 lipase gene in atherosclerosis

Abstract

One of the key components in the development of atherosclerosis is the metabolic imbalance of lipids. However, the role of epigenetic mechanisms in this process is studied insufficiently. The aim of our study was to characterize variability of the PNPLA2 lipase gene methylation in the cells of differently localized arteries and peripheral blood leukocytes in atherosclerosis. Materials and methods. Using bisulfite pyrosequencing, we performed the assessment of DNA methylation level in atherosclerotic plaques of coronary and carotid arteries, and in the wall of unaffected internal thoracic arteries, and in peripheral blood leukocytes of patients with atherosclerosis. Statistical data analysis was performed in R software environment. Results and conclusions. Cells of coronary atherosclerotic plaques were found to have higher levels of methylation in the promoter of PNPLA2 gene compared to unaffected wall of internal thoracic arteries. Methylation level of analyzed gene region in leukocytes of patients with atherosclerosis was associated with smoking and statins.