Filtration as a method for isolation of trophoblasts from maternal blood and analysis of single fetal cells
https://doi.org/10.1234/XXXX-XXXX-2016-1-38-42
Abstract
Non-invasive prenatal diagnosis of chromosomal disorders is a topical area of modern prenatal medicine. Identification of chromosomal status of the fetus avoiding invasive procedures perhaps by analyzing of trophoblasts circulating in peripheral maternal blood. In this article, we present the results of a model experiment for isolation of trophoblast cells from peripheral blood samples by filtration through polycarbonate filters. Isolation of single cells was performed by laser microdissection followed by a whole genome amplification step. Analysis of genetic material isolated cells was performed by comparative genomic hybridization.
About the Authors
E. V. Musatova
ФГБНУ «Медико-генетический научный центр»
Russian Federation
Russian Federation
A. V. Martynov
ФГБНУ «Медико-генетический научный центр»
Russian Federation
Russian Federation
Zh. G. Markova
ФГБНУ «Медико-генетический научный центр»
Russian Federation
Russian Federation
I. I. Vityazeva
ФГБУ «Эндокринологический научный центр» Министерства здравоохранения Российской Федерации
Russian Federation
Russian Federation
N. V. Shilova
ФГБНУ «Медико-генетический научный центр»
Russian Federation
Russian Federation
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Review
For citations:
Musatova E.V., Martynov A.V., Markova Zh.G., Vityazeva I.I., Shilova N.V. Filtration as a method for isolation of trophoblasts from maternal blood and analysis of single fetal cells. Medical Genetics. 2016;15(1):38-42. (In Russ.) https://doi.org/10.1234/XXXX-XXXX-2016-1-38-42
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