Cytogenetic damage in blood cells of lung cancer patients: rogue cell phenomenon

In the presented «case-control» study 890 residents of the Kemerovo Region subject to age, sex, ethnicity and smoking status were included. We formed two groups: 1) «Case» - 440 newly diagnosed lung cancer patients undergoing a medical treatment in the Kemerovo Regional Oncology Center (diagnosis «lung cancer» was determined by experienced doctors from the Kemerovo Regional Oncology based on the results of special medical examination); 2) «Control» - 450 healthy donors of the Kemerovo Regional Center of Blood Transfusion. The aim of this study was the analysis chromosomal aberrations (CAs) in lung cancer patients and healthy donors resident in the same territory. The following methods were used in our investigation: the routine method of lymphocytes cultivation and chromosomal aberration analysis. Statistical analysis were performed using nonparametric statistics (Mann-Whitney U Test for paired comparison of quantitative characteristics), logistic regression. It was determined that the CAs frequency (both chromatid- and chromosome-type aberrations) was significant increased in lung cancer patients compared to control group. Furthermore in 8 lung cancer patients were found Rogue cells that were contained polycentric, ring chromosomes and multiple pair dot fragments.

рак легкого, хромосомные аберрации, rogue cells, lung cancer, chromosomal aberrations, rogue cells

1. Heist RS, Sequist LV, Engelman JA. Genetic changes in squamous cell lung cancer: a review. J Thorac Oncol. 2012; 5: 924-957.

2. Пузырев ВП, Огородова ЛМ. Генетика бронхолегочных заболеваний. М: Атмосфера. 2010. 160с.

3. Hagmar L, Bonassi S, Stromberg U et al. Chromosomal aberrations in lymphocytes predict human сancer: a report from the European Study Group on Cytogenetic Biomarkers and Health (ESCH).Cancer Res. 1998; 58:4117-4121

4. Бочков НП, Чеботарев АН, Катосова ЛД и др. База данных для анализа количественных характеристик частоты хромосомных аберраций в культуре лимфоцитов периферической крови человека. Генетика. 2001; 37(4): 549-557.

5. Исламов ЗС, Гильдиева МС, Усманов РХ. Анализ цитогенетических изменений и их роль в ранней диагностике ретинобластомы. Практическая медицина. 2015; 1: 137-139.

6. Liou SH, Lung JC, Chen YH et al. Increased chromosome type chromosome aberration frequencies as biomarkers of cancer risk in a blackfoot endemic area. Cancer Res. 1999. 59(7): 1481-1484.

7. Bonassi S, Norppa H, Ceppi M et al. Chromosomal aberration frequency in lymphocytes predicts the risk of cancer: results from a pooled cohort study of 22 358 subjects in 11 countries. Carcinogenesis. 2008; 29(6): 1178-1183.

8. Vodenkova S, Polivkova Z, Musak L et al. Structural chromosomal aberrations as potential risk markers in incident cancer patients. Mutagenesis. 2015. 30(4): 557-563.

9. Vodicka P, Polivkova Z, Sytarova S et al. Chromosomal damage in peripheral blood lymphocytes of newly diagnosed cancer patients and healthy controls. Carcinogenesis. 2010. 31: 1238-1241.

10. Minina VI, Sinitsky MYu, Druzhinin VG et al. Chromosome aberrations in peripheral blood lymphocytes of lung cancer patients exposed to rad on and air pollution. Eur. J. Cancer Prevention. 2016; 25(4): 70-77.

11. Пилинская МА, Шеметун АМ, Дыбский СС и др. Выявление мультиаберрантных лимфоцитов при цито-генетическом обследовании различных групп людей, контактирующих с мутагенными факторами. Цитология и генетика. 1994. 28. (1): 27 - 32.

12. Jemal A, Bray F, Center M et al. Global cancer statistics. Cancer J. Clin. 2011; 61: 69-90.

13. Мун СА, Глушков АН. Расчет прогнозов заболеваемости раком легкого у мужчин в связи с техногенным загрязнением атмосферы в Кемеровской области. Гигиена и санитария. 2014; 2: 37-41.

14. Awa AA, Neel JV. Cytogenetic‘rogue’ cells: what is their frequency, origin, and evolutionary significance? Proc Natl Acad Sci USA. 1986; 83: 1021-1025.

15. National Research Council. Health Effects of Exposure to Radon (BEIR VI). Washington, DC: National Academy Press, 1999, 516р.

16. Schoenberg JB, Klotz JB, Wilcox HB et al. Case-control study of residential radon and lung cancer among New Jersey women. Cancer Res. 1990; 50:6520-6524

17. Druzhinin V, Bakanova M, Fucic A et al. Lymphocytes with multiple chromosomal damages in a large cohort of West Siberia residents: Results of long-term monitoring. Mutat Res. 2016; 784:1-7.

18. Lazutka JR, Neel JV, Major EO et al. High titers of antibodies to two human polyomaviruses, JCV and BKV, correlate with increased frequency of chromosomal damage in human lymphocytes. Cancer Lett. 1996; 109: 177-183.

19. Neel JV, Major EO, Awa AA et al. Hypothesis: Rogue cell-type chromosomal damage in lymphocytes is associated with infection with the JC human polyoma virus and has implications for oncogenesis. Proc. Nat. Acad. Sci. U.S.A. 1996; 93: 2690-2695.

20. Christiansen JJ, Rajasekaran AK. Reassessing epithelial to mesenchymal transition as a prerequisite for carcinoma invasion and metastasis. Cancer Res. 2006; 66: 8319-26.