DNA methylation of genes encoding matrix metalloproteinases and their inhibitors in normal and cancerous breast tissues

Matrix metalloproteinases and their inhibitors contribute to the regulation of cell-cell interactions and maintain tissue structure and function. We have analyzed DNA methylation at the promoter regions of 11 matrix metalloproteinases genes ( MMP2, MMP11, MMP14, MMP15, MMP16, MMP17, MMP21, MMP23B, MMP24, MMP25, MMP28 ), and 4 inhibitors of matrix metalloproteinases genes ( TIMP1, TIMP2, TIMP3, TIMP4 ). Collectively members of these families take part in regulation of matrix proteins degradation, signal transduction, adhesion, migration, cell differentiation, apoptosis and angiogenesis. Abnormal methylation frequencies at the promoter regions of genes MMP2, MMP23B, MMP24, MMP25, MMP28 in breast cancer accounted for 7,7%, 17%, 11,9%, 15,4% and 4,9% respectively. Promoter regions of genes TIMP1, TIMP4, MMP14 MMP21 were constitutively methylated in breast tissue.

внеклеточный матрикс, матриксные металлопротеиназы, ингибиторы матриксных металлопротеиназ, метилирование ДНК, extracellular matrix, matrix metalloproteinases, inhibitors of matrix metalloproteinases, DNA methylation

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