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Study of the effect of methylene blue on DNA breaks in Esсherichia coli cells using gel electrophoresis method
https://doi.org/10.25557/2073-7998.2025.08.110-111
Abstract
The method of gel electrophoresis of DNA was used to assess the extent of DNA damage in bacteria exposed to methylene blue (MB). Gel electrophoretic analysis of DNA integrity in Escherichia coli cells revealed that MB induces DNA breaks, with the level of fragmentation depending on its concentration.
Supporting agencies: State Task 125040704886-1
About the Authors
А. Saparova
Lomonosov Moscow State University
Russian Federation
Russian Federation
Ayna Saparova
119991; GSP-1, Leninskie Gory; Moscow
E. V. Igonina
Vavilov Institute of General Genetics Russian Academy of Sciences
Russian Federation
Russian Federation
119333; 3, Gubkina st.; Moscow
S. K. Abilev
Lomonosov Moscow State University; Vavilov Institute of General Genetics Russian Academy of Sciences
Russian Federation
Russian Federation
119991; GSP-1, Leninskie Gory; 119333; 3, Gubkina st.; Moscow
References
1. Igonina E.V., Marsova M.V. Abilev S.K. Lux-biosensory: skrining biologicheski aktivnyh soedinenij na genotoksichnost’ [Lux biosensors: screening of biologically active compounds for genotoxicity]. Ekologicheskaya genetika [Ecological genetics], 2016; (4): 52-62. (In Russian).
2. Igonina E.V., Saparova A. Izuchenie molekulyarno-geneticheskih mekhanizmov dejstviya metilenovogo sinego s primeneniem lux-biosensorov [Study of the molecular and genetic mechanisms of action of methylene blue using lux biosensors.]. Sovremennye tekhnologii: aktual’nye voprosy teorii i praktiki. Sbornik statej VII Mezhdunarodnoj nauchno-prakticheskoj konferencii. Penza [Assessment Modern technologies: current issues of theory and practice. Collection of articles of the VII International Scientific and Practical Conference. Penza], 2024; 17-20. (In Russian).
Review
For citations:
Saparova А., Igonina E.V., Abilev S.K. Study of the effect of methylene blue on DNA breaks in Esсherichia coli cells using gel electrophoresis method. Medical Genetics. 2025;24(8):110-111. (In Russ.) https://doi.org/10.25557/2073-7998.2025.08.110-111
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