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Analysis of CRISPR/Cas9 Off-Target Activity in a Yeast Model
https://doi.org/10.25557/2073-7998.2025.07.126-128
Abstract
Cas9-based genome editing methods may pose a risk when used in medicine because CRISPR/Cas9 systems are mutagenic, capable of introducing single- and double-strand breaks in DNA in both target and non-target regions of the genome, leading to unwanted mutations at the break site. The non-target activity of the editing complex is due to its ability to recognize 20-nucleotide sites in the genome that are not fully complementary to its guide RNA and introduce a double-strand break. In this study, we used the yeast Saccharomyces cerevisiae as a model organism to evaluate the effect of mismatches at different positions of the guide RNA on the editing efficiency of Cas9.
About the Authors
A. R. Shumega
Vavilov Institute of General Genetics, St. Petersburg Branch, Russian Academy of Sciences;
St. Petersburg State University, Department of Genetics and Biotechnology
Russian Federation
Russian Federation
17, Botanicheskaya st., St. Petersburg, Peterhof, 198504;
7-9, Universitetskaya emb., Saint Petersburg, 199034
D. M. Deviatkin
St. Petersburg State University, Department of Genetics and Biotechnology
Russian Federation
Russian Federation
7-9, Universitetskaya emb., Saint Petersburg, 199034
E. I. Stepchenkova
Vavilov Institute of General Genetics, St. Petersburg Branch, Russian Academy of Sciences;
St. Petersburg State University, Department of Genetics and Biotechnology
Russian Federation
Russian Federation
17, Botanicheskaya st., St. Petersburg, Peterhof, 198504;
7-9, Universitetskaya emb., Saint Petersburg, 199034
References
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2. Lada A.G., Stepchenkova E.I., Waisertreiger I.S., et al. Genome-wide mutation avalanches induced in diploid yeast cells by a base analog or an APOBEC deaminase. PLoS Genet. 2013;9(9):e1003736. doi: 10.1371/journal.pgen.1003736.
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Review
For citations:
Shumega A.R., Deviatkin D.M., Stepchenkova E.I. Analysis of CRISPR/Cas9 Off-Target Activity in a Yeast Model. Medical Genetics. 2025;24(7):126-128. (In Russ.) https://doi.org/10.25557/2073-7998.2025.07.126-128
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