Comparison of the efficacy of gene therapy based on viral or non-viral vectors with the BMP2 gene for bone regeneration

Abstract

Gene therapy by local delivery of target genes using gene-activated matrices (GAM) for the treatment of patients with bone diseases represents a promising approach. The aim of the work is a comparative study of the osteoinductive effect of GAM based on adenoviral or plasmid vectors with the BMP2 gene in vitro and in vivo. Using RT-PCR, ELISA, alizarin red staining and micro-CT, it was shown that the developed GAM provide delivery of the target gene to cells, stimulate osteogenic differentiation and reparative neoosteogenesis during intraosseous implantation.