

Current challenges of genotoxicity testing
https://doi.org/10.25557/2073-7998.2025.07.37-39
Abstract
There are two challenges that need to be addressed today in the genotoxicity testing. The first one includes improvement of in vitro testing methodology and development of high-throughput test systems for genotoxicity screening. The second one is determined by the necessity to develop new methods to register gene mutations in vivo, including genotoxicity in germ cells. Current methodological achievements promising for solving the above tasks are considered.
About the Authors
A. K. ZhanataevRussian Federation
8, Baltiyskaya st., Moscow, 125315
A. D. Durnev
Russian Federation
8, Baltiyskaya st., Moscow, 125315
References
1. Durnev A.D., Zhanataev A.K., Eremina N.V. Geneticheskaya toksikologiya. M.: Mittel’ Press [Genetic toxicology. M.: Mittel Press], 2022. 286 p. (In Russ.)
2. Groff K., Evans S.J., Doak S.H., et al. In vitro and integrated in vivo strategies to reduce animal use in genotoxicity testing. Mutagenesis. 2021;36(6):389-400.
3. Martus H.J., Froetschl R., Gollapudi B., et al. Summary of major conclusions from the 7th International Workshop on Genotoxicity Testing (IWGT), Tokyo, Japan. Mutat Res Genet Toxicol Environ Mutagen. 2020; 852:503134
4. Štampar M., Žegura B. In vitro hepatic 3D cell models and their application in genetic toxicology: A systematic review. Mutat Res Genet Toxicol Environ Mutagen. 2024;900:503835.
5. Durnev A.D., Zhanataev A.K. Aktual’nyye aspekty geneticheskoy toksikologii lekarstvennykh sredstv [Relevant Aspects of Drug Genetic Toxicology]. Vedomosti Nauchnogo tsentra ekspertizy sredstv meditsinskogo primeneniya. Regulyatornyye issledovaniya i ekspertiza lekarstvennykh sredstv [Bulletin of the Scientific Centre for Expert Evaluation of Medicinal Products. Regulatory Research and Medicine Evaluation]. 2022;12(1):90-109. (In Russ.)
6. Walmsley R.M., Tate M. The GADD45a-GFP GreenScreen HC assay. Methods Mol Biol. 2012; 817:231-50.
7. Zeller A., Pfuhler S., Albertini S., et al. A critical appraisal of the sensitivity of in vivo genotoxicity assays in detecting human carcinogens. Mutagenesis. 2018;33(2):179-193.
8. Marchetti F., Cardoso R., Chen C.L., et al. Error-corrected next generation sequencing – promises and challenges for genotoxicity and cancer risk assessment. Mutat Res Rev Mutat Res. 2023;792:108466.
Review
For citations:
Zhanataev A.K., Durnev A.D. Current challenges of genotoxicity testing. Medical Genetics. 2025;24(7):37-39. (In Russ.) https://doi.org/10.25557/2073-7998.2025.07.37-39