Analysis of the expression of long non-coding RNAs in HPV infection

Abstract

HPV types of high oncogenic risk are recognized as the cause of cervical cancer. The literature notes differences in the expression of certain lncRNAs in cells affected by HPV-associated cancer compared with uninfected ones, which makes them potentially good markers for predicting cancer development. The aim of the study was to evaluate the level of transcription of the DINO and HOST2 lncRNAs genes in epithelial cells of the cervical canal of women with HPV infection with a clinically significant viral load. RT-PCR was used to obtain data on the relative expression levels of DINO and HOST2 in the women cells in two groups (HPV infected and control group). The relative level of the lncRNAs transcription is the same in these groups of cells. A direct correlation was found between the expression levels of HOST2 and DINO. It was found that the expression levels of DINO and HOST2 lncRNAs correlate with the mRNA levels of protein-coding genes TP53 and TP73 under high viral load.