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Using CRISPR/Cas technology to generate A549 human lung cancer subline with knockout of the E2F1 gene
https://doi.org/10.25557/2073-7998.2024.11.40-46
Abstract
Despite a significant number of studies devoted to the transcription factor E2F1, its functional role in cellular processes remains ambiguous. Depending on the context, E2F1 can either support cell survival or initiate apoptosis. The present work is devoted to the possibility of using E2F1 as a therapeutic target for the combined treatment of malignant neoplasms, including through the use of inhibitors. However, the available data also indicate a potentially opposite effect of E2F1, which can negatively affect the effectiveness of therapy. This emphasizes the relevance of an in-depth study of the functional activity of E2F1 in various conditions. Transcription factors of the E2F family, including E2F1, demonstrate both overlapping functions and unique properties inherent in its individual members. Suppression of the expression of individual representatives of the family makes it possible to more accurately assess their contribution to key cellular processes. As part of the study, a subline of A549 lung cancer cells with a knockout of the E2F1 gene, carried out using CRISPR/Cas technology, was developed. Based on this cell model, experiments are planned to be carried out aimed at studying the role of E2F1 in various conditions, including responses to chemotherapeutic effects
About the Authors
М. А. Zamkova
Institute of Gene Biology, Russian Academy of Sciences; National Medical Research Center of Oncology named after N.N. Blokhin of the Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
34/5, Vavilova st., Moscow, 119334;
23, Kashirskoe shosse, Moscow, 115522
D. B. Kazansky
National Medical Research Center of Oncology named after N.N. Blokhin of the Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
23, Kashirskoe shosse, Moscow, 115522
V. V. Tatarskiy
Institute of Gene Biology, Russian Academy of Sciences
Russian Federation
Russian Federation
34/5, Vavilova st., Moscow, 119334
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Review
For citations:
Zamkova М.А., Kazansky D.B., Tatarskiy V.V. Using CRISPR/Cas technology to generate A549 human lung cancer subline with knockout of the E2F1 gene. Medical Genetics. 2024;23(11):40-46. (In Russ.) https://doi.org/10.25557/2073-7998.2024.11.40-46
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