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Pilot association of common NOS3 variants with Preeclampsia risk in Russian pregnant women from Rostov region
https://doi.org/10.25557/2073-7998.2024.06.11-19
Abstract
Preeclampsia (PE) is a severe medical condition affecting pregnant women, often developing after 20 weeks, and is the most common serious disorder during pregnancy, contributing to 2%-8% of pregnancy-related complications worldwide. The goal of this study is to investigate the association of two genetic variants in NOS3 (-786T>C; rs2070744) and (894G>T; rs1799983) with the risk of developing PE in Russian pregnant women from Rostov region. The case–control study involved 106 pregnant women (46 pregnant women with PE and 60 healthy controls). Genotyping of the selected NOS3 genetic variants (-786T>C) and (894G>T) was done using allele specific RT-PCR. Multifactor dimensionality reduction (MDR) was used to assess the relationship between the SNP-SNP interaction and risk of developing PE. In addition, we analyzed the linkage disequilibrium (LD), and haplotypes association for the selected SNPs in the studied population. According to our results, both NOS3 polymorphisms were statistically significant (P=0.004 and P=0.045, respectively). The NOS3 -786(TT) genotype (OR=0.25 95% CI [0.11- 0.58]) and the NOS3 894(GG) genotype (OR=0.38 95% CI [0.17- 0.84]) were associated with low risk of developing PE. While -786(TC) genotype (OR=4.17 95% CI [1.76-9.85]), 894(GT) (OR=2.48 95% CI [1.10-5.63]) and dominant model of NOS3 (-786T>C) TC+CC (OR=3.97 95% CI [1.72-9.14]) as well as the dominant model GT+TT of NOS3 (894G>T) (OR=2.64 95% CI [1.20-5.82]) were associated with increased risk of PE. In addition, the polymorphism -786(C) allele (OR=2.21 95% CI [1.23-3.98]) and polymorphism 894(T) allele (OR=2.14 95% CI [1.15-4.01]) were both associated with an increased risk of PE. According to the MDR results, the SNP-SNP interaction was statistically significant (P=0.0003). Moreover, two haplotypes of NOS3 gene variants -786C*894G (OR=3.01 95% CI [1.15- 7.86]; P=0.027) and -786C*894T (OR=3.03 95% CI [1.27-7.23]; P=0.014) were shown to be statistically related with an elevated risk of PE.
Supporting agencies: This study was funded by the Ministry of Science and Higher Education of the Russian Federation grant № FENW-2023-0018.
About the Authors
N. N. Alayasa
Academy of Biology and Biotechnology named after D I Ivanovsky, Southern Federal University
Russian Federation
Russian Federation
194/1 Stachki Avenue, Rostov on Don 344090
T. P. Shkurat
Academy of Biology and Biotechnology named after D I Ivanovsky, Southern Federal University; Medical Center “Nauka”
Russian Federation
Russian Federation
194/1 Stachki Avenue, Rostov on Don 344090
23A Zagorskaya, Rostov-on-Don 344034
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Review
For citations:
Alayasa N.N., Shkurat T.P. Pilot association of common NOS3 variants with Preeclampsia risk in Russian pregnant women from Rostov region. Medical Genetics. 2024;23(6):11-19. (In Russ.) https://doi.org/10.25557/2073-7998.2024.06.11-19
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