Methylation level analysis of putative regulatory region of MIR100 gene in carotid atherosclerosis by targeted bisulfite sequencing
https://doi.org/10.25557/2073-7998.2023.01.43-46
Abstract
Expression of miR-100 is increased in unstable carotid atherosclerotic plaques. It can be associated with CpG sites methylation variability in the MIR100 gene region and its adjacent regulatory regions.
The aim of our work was to analyze the association of DNA methylation level in the putative regulatory region of MIR100 gene with advanced carotid atherosclerosis using targeted bisulfite sequencing.
We analyzed DNA samples of paired vessel tissues and blood leukocytes of patients with advanced atherosclerosis of the carotid arteries (n = 19), as well as blood leukocytes of healthy individuals (n = 18). As a result, we revealed tissue-specific methylation pattern of the chr11:122,024,891-122,025,506 region (GRCh37/hg19 genome assembly) including E-box (see Chen D. et al, 2014 for more information). The methylation level in unaffected carotid arteries was decreased compared to both veins (p < 0.001) and peripheral blood leukocytes (p < 0.007). Significant hypomethylation was observed in atherosclerotic carotid plaques relative to unaffected carotid arteries, but the level of DNA methylation in blood leukocytes of patients with atherosclerosis did not differ from that in blood leukocytes of the control group. Our findings suggest tissue-specific methylation of the putative regulatory region of MIR100 gene (E-box) and its possible association to atherosclerosis. Nevertheless, the methylation of the chr11:122,024,891-122,025,506 region cannot be used as a diagnostic biomarker of atherosclerosis.
About the Authors
I. A. KorolevaRussian Federation
634050
10, Nab. r. Ushaiki
Tomsk
A. A. Zarubin
Russian Federation
634050
10, Nab. r. Ushaiki
Tomsk
N. P. Babushkina
Russian Federation
634050
10, Nab. r. Ushaiki
Tomsk
D. E. Gomboeva
Russian Federation
634050
10, Nab. r. Ushaiki
Tomsk
E. F. Muslimova
Russian Federation
634012
111 a, Kyevskaya str.
Tomsk
M. S. Kuznetsov
Russian Federation
634012
111 a, Kyevskaya str.
Tomsk
B. N. Kozlov
Russian Federation
634012
111 a, Kyevskaya str.
Tomsk
M. S. Nazarenko
Russian Federation
634050
10, Nab. r. Ushaiki
Tomsk
References
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4. Chen D., Sun Y., Yuan Y. et al. miR-100 induces epithelial-mesen-chymal transition but suppresses tumorigenesis, migration and invasion. PLoS genetics. 2014; 10 (2); e1004177.
Review
For citations:
Koroleva I.A., Zarubin A.A., Babushkina N.P., Gomboeva D.E., Muslimova E.F., Kuznetsov M.S., Kozlov B.N., Nazarenko M.S. Methylation level analysis of putative regulatory region of MIR100 gene in carotid atherosclerosis by targeted bisulfite sequencing. Medical Genetics. 2023;22(1):43-46. (In Russ.) https://doi.org/10.25557/2073-7998.2023.01.43-46