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Methylation level analysis of putative regulatory region of MIR100 gene in carotid atherosclerosis by targeted bisulfite sequencing

https://doi.org/10.25557/2073-7998.2023.01.43-46

Abstract

   Expression of miR-100 is increased in unstable carotid atherosclerotic plaques. It can be associated with CpG sites methylation variability in the MIR100 gene region and its adjacent regulatory regions.

   The aim of our work was to analyze the association of DNA methylation level in the putative regulatory region of MIR100 gene with advanced carotid atherosclerosis using targeted bisulfite sequencing.

   We analyzed DNA samples of paired vessel tissues and blood leukocytes of patients with advanced atherosclerosis of the carotid arteries (n = 19), as well as blood leukocytes of healthy individuals (n = 18). As a result, we revealed tissue-specific methylation pattern of the chr11:122,024,891-122,025,506 region (GRCh37/hg19 genome assembly) including E-box (see Chen D. et al, 2014 for more information). The methylation level in unaffected carotid arteries was decreased compared to both veins (p < 0.001) and peripheral blood leukocytes (p < 0.007). Significant hypomethylation was observed in atherosclerotic carotid plaques relative to unaffected carotid arteries, but the level of DNA methylation in blood leukocytes of patients with atherosclerosis did not differ from that in blood leukocytes of the control group. Our findings suggest tissue-specific methylation of the putative regulatory region of MIR100 gene (E-box) and its possible association to atherosclerosis. Nevertheless, the methylation of the chr11:122,024,891-122,025,506 region cannot be used as a diagnostic biomarker of atherosclerosis.

About the Authors

I. A. Koroleva
Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Sciences
Russian Federation

634050

10, Nab. r. Ushaiki

Tomsk



A. A. Zarubin
Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Sciences
Russian Federation

634050

10, Nab. r. Ushaiki

Tomsk



N. P. Babushkina
Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Sciences
Russian Federation

634050

10, Nab. r. Ushaiki

Tomsk



D. E. Gomboeva
Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Sciences
Russian Federation

634050

10, Nab. r. Ushaiki

Tomsk



E. F. Muslimova
Cardiology Research Institute, Tomsk National Research Medical Centre, Russian Academy of Sciences
Russian Federation

634012

111 a, Kyevskaya str.

Tomsk



M. S. Kuznetsov
Cardiology Research Institute, Tomsk National Research Medical Centre, Russian Academy of Sciences
Russian Federation

634012

111 a, Kyevskaya str.

Tomsk



B. N. Kozlov
Cardiology Research Institute, Tomsk National Research Medical Centre, Russian Academy of Sciences
Russian Federation

634012

111 a, Kyevskaya str.

Tomsk



M. S. Nazarenko
Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Sciences
Russian Federation

634050

10, Nab. r. Ushaiki

Tomsk



References

1. Cipollone F., Felicioni L., Sarzani R. et al. A unique microRNA signature associated with plaque instability in humans. Stroke.2011; 42 (9): 2556-2563.

2. Maitrias P., Metzinger-Le Meuth V., Massy Z. A. et al. MicroRNA deregulation in symptomatic carotid plaque. Journal of vascular surgery.2015; 62 (5); 1245-1250.e1.

3. Morales S., Monzo M., Navarro A. Epigenetic regulation mechanisms of microRNA expression. Biomolecular concepts. 2017; 8 (5-6); 203-212.

4. Chen D., Sun Y., Yuan Y. et al. miR-100 induces epithelial-mesen-chymal transition but suppresses tumorigenesis, migration and invasion. PLoS genetics. 2014; 10 (2); e1004177.


Review

For citations:


Koroleva I.A., Zarubin A.A., Babushkina N.P., Gomboeva D.E., Muslimova E.F., Kuznetsov M.S., Kozlov B.N., Nazarenko M.S. Methylation level analysis of putative regulatory region of MIR100 gene in carotid atherosclerosis by targeted bisulfite sequencing. Medical Genetics. 2023;22(1):43-46. (In Russ.) https://doi.org/10.25557/2073-7998.2023.01.43-46

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ISSN 2073-7998 (Print)