Cellular stress blocks the transcription of satellite III on the first chromosome and reduces the content of satellite III in the DNA of cultured skin fibroblasts in patients with schizophrenia
https://doi.org/10.25557/2073-7998.2022.12.23-25
Abstract
It was previously found that in the blood leukocytes of patients with schizophrenia, compared to the control, the content of the tandem repeat - the subfraction of satellite III (SatIII), which is part of the pericentromeric heterochromatin of the first chromosome (region 1q12) - is significantly reduced. One of the reasons behind the low repeat level in the DNA of patients may be the low level of SatIII transcription under stress conditions. The study shows that cellular stress in cultured skin fibroblasts of patients with schizophrenia and healthy people, caused by an increase in cultivation temperature, significantly blocks the transcription of SatIII (1q12) and increases the transcription level of SatIII on the 9th chromosome tenfold. For cells of patients with schizophrenia, a decrease in the content of SatIII(1q12) in DNA positively correlates with a decrease in the amount of SatIII RNA. In control cells, the content of SatIII in DNA did not change under stress. Blocking of SatIII transcription under stress conditions can potentially be one of the reasons for the decrease in the content of the SatIII repeat in the DNA of patients with schizophrenia.
About the Authors
N. A. Salimova
Research Centre for Medical Genetics
Russian Federation
Russian Federation
N. N. Veiko
Research Centre for Medical Genetics
Russian Federation
Russian Federation
E. S. Ershova
Research Centre for Medical Genetics
Russian Federation
Russian Federation
N. V. Zaharova
N.A. Alexeev Clinical Psychiatric Hospital No. 1
Russian Federation
Russian Federation
S. V. Kostyuk
Research Centre for Medical Genetics
Russian Federation
Russian Federation
References
1. Imamura A., Morimoto Y., Ono S., Kurotaki N., Kanegae S., Yamamoto N., Kinoshita H., Tsujita T., Okazaki Y., Ozawa H. Genetic and environmental factors of schizophrenia and autism spectrum disorder: insights from twin studies. J Neural Transm (Vienna). 2020 Nov;127(11):1501-1515. doi: 10.1007/s00702-020-02188-w
2. Ermakov E.A., Dmitrieva E.M., Parshukova D.A., Kazantseva D.V., Vasilieva A.R., Smirnova L.P. Oxidative Stress-Related Mechanisms in Schizophrenia Pathogenesis and New Treatment Perspectives. Oxid Med Cell Longev. 2021 Jan 23;2021:8881770. doi: 10.1155/2021/8881770
3. Ershova E.S., Malinovskaya E.M., Golimbet V.E., Lezheiko T.V., Zakharova N.V., Shmarina G.V., Veiko R.V., Umriukhin P.E., Kostyuk G.P., Kutsev S.I,. Izhevskaya V.L., Veiko N.N., Kostyuk S.V. Copy number variations of satellite III (1q12) and ribosomal repeats in health and schizophrenia. Schizophr Res. 2020 Sep;223:199-212. doi: 10.1016/j.schres.2020.07.022
4. Bersani F., Lee E., Kharchenko P.V., Xu A.W., Liu M., Xega K., MacKenzie O.C., Brannigan B.W., Wittner B.S., Jung H., Ramaswamy S., Park P.J., Maheswaran S., Ting D.T., Haber D.A. Pericentromeric satellite repeat expansions through RNA-derived DNA intermediates in cancer. Proc Natl Acad Sci U S A. 2015 Dec 8;112(49):15148-53. doi: 10.1073/pnas.1518008112
5. Valgardsdottir R., Chiodi I., Giordano M., Rossi A., Bazzini S., Ghigna C., Riva S., Biamonti G. Transcription of Satellite III non-coding RNAs is a general stress response in human cells. Nucleic Acids Res. 2008 Feb;36(2):423-34. doi: 10.1093/nar/gkm1056
Review
For citations:
Salimova N.A., Veiko N.N., Ershova E.S., Zaharova N.V., Kostyuk S.V. Cellular stress blocks the transcription of satellite III on the first chromosome and reduces the content of satellite III in the DNA of cultured skin fibroblasts in patients with schizophrenia. Medical Genetics. 2022;21(12):23-25. (In Russ.) https://doi.org/10.25557/2073-7998.2022.12.23-25
Views:
225
Email this article 