Influence of ibuprofen on adenoviral transduction of multipotent mesenchymal stromal cells
https://doi.org/10.25557/2073-7998.2022.12.4-9
Abstract
One of the main problems in the development of gene therapy methods based on adenovirus constructs is the immune response to the introduction of viral particles. It is proposed to use the non-steroidal anti-inflammatory drug ibuprofen to reduce the immune response. Ibuprofen at high concentrations has a dose-dependent toxic effect on cultures of multipotent mesenchymal stromal cells (MSCs). It was determined that at concentration of 0,125 mg/ml ibuprofen does not cause cell death, and also has a positive effect on adenovirus transduction of MSCs: 48,47±2,06% of transduced cells were detected 3 days after infection with 320 TCID50/ml Ad-GFP, and when 0,125 mg/ml Ibu was added - 60,07±1,64% (p < 0,05). Ibuprofen can be used in gene therapy based on adenoviral transduction to reduce the immune response to local exposure high doses of viral vectors.
About the Authors
T. B. Bukharova
Research Centre for Medical Genetics
Russian Federation
Russian Federation
I. A. Nedorubova
Research Centre for Medical Genetics
Russian Federation
Russian Federation
V. O. Mokrousova
Research Centre for Medical Genetics; Central Research Institute of Dental and Maxillofacial Surgery
Russian Federation
Russian Federation
A. Yu. Meglei
Research Centre for Medical Genetics; Central Research Institute of Dental and Maxillofacial Surgery
Russian Federation
Russian Federation
A. V. Vasilyev
Research Centre for Medical Genetics; Central Research Institute of Dental and Maxillofacial Surgery
Russian Federation
Russian Federation
D. V. Goldstein
Research Centre for Medical Genetics
Russian Federation
Russian Federation
References
1. Недорубова И.А., Бухарова Т.Б., Васильев А.В. и др. Невирусная доставка гена BMP2 для регенерации костной ткани. Гены и Клетки. 2020;15(4):33-39
2. Warnock J.N., Daigre C., Al-Rubeai M.Introduction to viral vectors. Viral vectors for gene therapy. 2011;1-25
3. Hartman Z.C., Appledorn D.M., Amalfitano A. Adenovirus vector induced innate immune responses: impact upon efficacy and toxicity in gene therapy and vaccine applications. Virus res. 2008;132(1-2):1-14
4. Bushra R., Aslam N. An overview of clinical pharmacology of ibuprofen. Oman Med J. 2010;25(3):155
5. Tolba R. Nonsteroidal anti-inflammatory drugs (NSAIDs). Treatment of Chronic Pain Conditions. 2017;77-79
6. Волков А.В., Антонов Е.Н., Васильев А.В. и др. Влияние противовоспалительных препаратов на регенерацию костной ткани при трансплантации мультипотентных мезенхимальных стромальных клеток. Биомедицина. 2014;(4):17-24
7. Бухарова Т.Б., Волков А.В., Антонов Е.Н. и др. Тканеинженерная конструкция на основе мультипотентных мезенхимальных стромальных клеток жировой ткани, полилактидных носителей и тромбоцитарного геля. Клеточная трансплантология и тканевая инженерия. 2013;8(4):61-68
8. Bukharova T.B., Logovskaya L.V., Vikhrova E.B. et al. Аdenoviral transduction of multipotent mesenchymal stromal cells from human adipose tissue with bone morphogenetic protein BMP-2 gene. Bull Exp Biol Med. 2013;156(1):122-126
Review
For citations:
Bukharova T.B., Nedorubova I.A., Mokrousova V.O., Meglei A.Yu., Vasilyev A.V., Goldstein D.V. Influence of ibuprofen on adenoviral transduction of multipotent mesenchymal stromal cells. Medical Genetics. 2022;21(12):4-9. (In Russ.) https://doi.org/10.25557/2073-7998.2022.12.4-9
Views:
389
Email this article 