Molecular markers of T2DM in obesity: expression profile of inflammatory response genes in peripheral blood mononuclear cells

Type 2 diabetes mellitus (T2DM) is a metabolic multifactorial, genetically determined, life-threatening disease. The development of complications is associated with a chronic immuno-inflammatory process and with the formation of immune complexes. The aim of the study was to analyze the expression profile of a complex of functionally interconnected genes (cytokines, chemokines, and other key molecules of the inflammatory response) in peripheral blood mononuclear cells in patients with T2DM (N = 10) and in the control group (N = 10). Patients and controls were matched by gender, age, and belonged to the Tatars ethnic group. The expression profile of 84 immune response genes was studied by OT-PCR on a BioRad CFX96TM instrument using the Human Cytokines & Chemokines PCR Array RT2 Profiler PCR Arrays kit (Qiagen). Significant changes in expression (p <0.05) in the general group of patients with T2DM compared with the control were found for the FASLG, IL16, OSM, CSF1, CXCL16, BMP6 genes. Moreover, the expression profile of these genes in patients with type 2 diabetes is characterized by an increase in the level from 2.1 to 6.7 times in comparison with healthy individuals. The gene expression profile of CXCL8, CXCL1, CXCL2, CXCL10, CCL2, IL1RN is characterized by a decrease in the level from 2.1 to 8.78 times in comparison with healthy individuals. Significant statistical differences with a decrease in expression were obtained for the genes IL1RN (fold-change (FCh) = - 3.31, p = 0.013), CXCL1 (FCh = -2.74, p = 0.027), CXCL2 (FCh = -2.66, p = 0.038) . The most significant increase in transcriptional activity was shown for the genes IL16 (FCh = 6.72, p = 0.037) and FASLG (FCh = 2.83 p = 0.034) among patients with type 2 diabetes compared with the control group.

сахарный диабет 2 типа, системное воспаление, профиль экспрессии, молекулярные маркеры, type 2 diabetes mellitus, systemic inflammation, expression profile, molecular markers