Analysis of IL-10 cytokine level in blood serum of psoriasis and psoriatic arthritis patients with different genotypes of polymorphism С-597А of IL10 gene

Abstract

The purpose of the study. To estimate the concentration of IL-10 in blood serum of patients with psoriasis and psoriatic arthritis moderate severity, the carriers of different genotypes of the C-597А polymorphism of the IL10 gene. Materials and methods. The object of the study were psoriasis (PS, n = 39), and psoriatic arthritis (PsA, n = 41) patients and healthy blood donors (control, n = 94), Caucasians in three generations. The study included PS and PsA patients of medium disease severity. The serum concentration of IL-10 was determined by ELISA. IL10 gene polymorphism was studied by PCR-RFLP analysis. Results. The concentration of IL-10 in blood serum of PS patients, the carriers of C/A and A/A genotypes, significantly lower in comparison with the carriers of these genotypes in groups of PsA patients and control (р_1,2 = 0,02; р_1,3 = 0,003). The concentration of IL-10 in blood serum of PsA patients, carriers of the genotype C/C , significantly lower in comparison with carriers of this genotype in the control group (p_2,3 = 0,04) and carriers of genotype C/A and A/A group of patients with PsA (p = 0,04). Conclusions. Statistically significant low level of IL-10 in blood serum in PS and PsA of moderate severity depending on the presence of a particular genotype of polymorphism C-597А of IL10 gene revealed: in PS - patients with genotypes C/A and A/A , in PsA - patients with genotype C/C .