DNA methylation of the integrin, nidogen and dystroglycan genes in normal and breast carcinoma tissues

Transmembrane cellular receptors and extracellular matrix components play a fundamental role in regulation of cellular activity, and providing concerted integration of the cells in the tissue structure. We have analyzed DNA methylation at the promoter regions of eight integrin genes ( ITGA1, ITGA2, ITGA3, ITGA4, ITGA6, ITGA7, ITGA9, ITGB1 ), two nidogen genes and a dystroglycan gene in normal breast tissue, as well as in the breast carcinoma samples. Integrin and dystroglycan are transmembrane receptors; nidogens are components of the extracellular matrix. The protein products of these genes are able to interact with proteins of the basement membrane LAMA1, LAMA2 and LAMB1; abnormal methylation of the LAMA1 , LAMA2 and LAMB1 genes in breast cancer had been described in our previous publications. Abnormal methylation frequencies at the promoter regions of genes ITGA1 , ITGA4 , ITGA7 , ITGA9 , NID1 and NID2 in breast cancer accounted for 13.3%, 29.3%, 3.3%, 40.6%, 37.3%, 39.3%, respectively. In our study cohort, DNA methylation at promoter regions of the ITGA4 , ITGA9 and NID1 genes was enriched in HER2 positive tumors, a highly aggressive form of breast cancer.

внеклеточный матрикс, интегрины, нидогены, дистрогликан, метилирование ДНК, инвазия опухоли, рак молочной железы, extracellular matrix, integrin, nidogen, dystroglycan, DNA methylation, tumor invasion, breast cancer

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