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Identification of population characteristics of the decidual cells transcriptome in severe preeclampsia
https://doi.org/10.25557/2073-7998.2022.07.15-18
Abstract
Decidualization defects in early gestation are known to lead to fetal growth retardation, miscarriage, or various obstetric complications. The aim is to reveal the transcriptomic profile of decidual cells (DC) in severe preeclampsia in Russians and Buryats. A full-transcriptomic analysis of placenta DC was carried out in representatives of two ethnic groups (Russians (n=4) and Buryats (n=4)). Single DC were obtained using laser microdissection technology and thin stained section preparations. When comparing expression profiles of DC in the examined groups, statistically significant differences (FDR<0.05) were revealed for 61 transcripts. Most of the identified differentially expressed genes (DEGs) were characterized by overexpression in Russian samples, while only 15 genes showed a high level of expression in Buryats. The results obtained indicate that the pathways of the innate immunity system associated with the activity of interferon proteins predominate in the differentiation of the DC transcriptomic profile of Russian and Buryat women with preeclampsia, which was confirmed not only by functional annotation of the list of identified DEG genes, but also by network analysis of data.
About the Authors
A. A. Babovskaya
Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Sciences
Russian Federation
Russian Federation
E. A. Trifonova
Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Sciences
Russian Federation
Russian Federation
V. N. Serebrova
Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Sciences
Russian Federation
Russian Federation
M. G. Svarovskaya
Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Sciences
Russian Federation
Russian Federation
A. A. Zarubin
Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Sciences
Russian Federation
Russian Federation
L. V. Rychkova
Scientific Center for Family Health and Human Reproduction Problems
Russian Federation
Russian Federation
V. A. Stepanov
Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Sciences
Russian Federation
Russian Federation
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Review
For citations:
Babovskaya A.A., Trifonova E.A., Serebrova V.N., Svarovskaya M.G., Zarubin A.A., Rychkova L.V., Stepanov V.A. Identification of population characteristics of the decidual cells transcriptome in severe preeclampsia. Medical Genetics. 2022;21(7):15-18. (In Russ.) https://doi.org/10.25557/2073-7998.2022.07.15-18
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