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Epigenetic regulation of SNCA gene expression in Parkinson’s disease
https://doi.org/10.25557/2073-7998.2020.04.96-98
Abstract
The aim of this study was to assess the level of mRNA and protein of the SNCA, DNMT1 genes, as well as intron 1 methylation of the SNCA gene in CD45 + peripheral blood cells of patients with sporadic PD and control individuals. For the first time, a decrease in the concentration of DNMT1 protein in CD45 + peripheral blood cells from PD patients compare to controls was revealed. An increase in DNMT1 gene expression in PD patients compare to controls was found. No differences in intron 1 methylation of the SNCA gene in CD45 + peripheral blood cells was found between PD patients and controls. An inverse correlations between methylation level of 21, 22 CpG island in intron1 of SNCA gene and mRNA SNCA gene and alpha-synuclein protein level were found. The study suggests the involvement of DNMT1 in the pathogenesis of PD and the lack of association of PD with intron 1 methylation of the SNCA gene.
About the Authors
A. . Emelyanov
First Pavlov State Medical University of St. Petersburg
Russian Federation
Russian Federation
A. . Lavrinova
Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»
Russian Federation
Russian Federation
N. . Melnikova
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Russian Federation
Russian Federation
A. . Dmitriev
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Russian Federation
Russian Federation
I. . Miliukhina
First Pavlov State Medical University of St. Petersburg
Russian Federation
Russian Federation
A. . Timofeeva
First Pavlov State Medical University of St. Petersburg
Russian Federation
Russian Federation
E. . Litusova
Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»
Russian Federation
Russian Federation
P. . Gagarina
Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»
Russian Federation
Russian Federation
O. . Berkovich
First Pavlov State Medical University of St. Petersburg
Russian Federation
Russian Federation
S Pchelina
First Pavlov State Medical University of St. Petersburg
Russian Federation
Russian Federation
Review
For citations:
Emelyanov A., Lavrinova A., Melnikova N., Dmitriev A., Miliukhina I., Timofeeva A., Litusova E., Gagarina P., Berkovich O., Pchelina S. Epigenetic regulation of SNCA gene expression in Parkinson’s disease. Medical Genetics. 2020;19(4):96-98. (In Russ.) https://doi.org/10.25557/2073-7998.2020.04.96-98
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