medgenМедицинская генетикаMedical Genetics2073-7998Publishing House «Genius Media» LLC10.25557/2073-7998.2018.08.48-52medgen-526Research ArticleКРАТКОЕ СООБЩЕНИЕBRIEF REPORTИсследование взаимосвязи полиморфных вариантов генов VEGF и MUC2 с риском развития эндометриозаInvestigation of the interrelation between polymorphisms of VEGF and MUC2 genes with a risk of developing endometriosisСкуратовскаяД. А.SkuratovskaiaD. A.DariaSK@list.ruЮроваК. А.YurovaK. A.noemail@neicon.ruКуликоваН. В.KulikovaN. V.noemail@neicon.ruАфанасьеваМ. В.AfanasievaM. V.noemail@neicon.ruСедневО. В.SednevO. V.noemail@neicon.ruЦивьянБ. Л.TsivyanB. L.noemail@neicon.ruЛитвиноваЛ. С.LitvinovaL. S.noemail@neicon.ruФГАОУ ВО «Балтийский федеральный университет имени Иммануила Канта» Министерства образования и науки Российской ФедерацииРоссияLaboratory of Immunology and Cellular Biotechnology IKBFURussian FederationСПб ГБУЗ «Городская больница №40»РоссияSt. Petersburg City Clinical Hospital No. 40Russian FederationГБУЗ Калининградской области «Городская клиническая больница скорой медицинской помощи» Минздрава РоссииРоссияGynecological Department of BSMPRussian Federation2018081020181784852Copyright © Скуратовская Д.А., Юрова К.А., Куликова Н.В., Афанасьева М.В., Седнев О.В., Цивьян Б.Л., Литвинова Л.С., 20182018Скуратовская Д.А., Юрова К.А., Куликова Н.В., Афанасьева М.В., Седнев О.В., Цивьян Б.Л., Литвинова Л.С.Skuratovskaia D.A., Yurova K.A., Kulikova N.V., Afanasieva M.V., Sednev O.V., Tsivyan B.L., Litvinova L.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.medgen-journal.ru/jour/article/view/526

Целью исследования явился поиск ассоциации полиморфных вариантов +936C/T и +460C/T гена VEGF и полиморфных вариантов C(-15161)T (rs10902088) и T(-12150)C (rs10794288) гена MUC2 с риском развития наружного генитального эндометриоза в славянской популяции Северо-Западного федерального округа. В исследование включено 60 пациенток (средний возраст 32,1 ± 12,4 года) с диагнозом наружный генитальный эндометриоз и 76 здоровых доноров (средний возраст 29,6 ± 9,7 года). Генотипирование проводилось методом ПЦР в режиме реального времени с использованием амплификатора LightCycler 480 Instrument II («Roche», Швейцария) и наборов для определения полиморфных вариантов VEGF T(-460)C (rs833061), VEGF C936T (rs3025039), MUC2 C(-15161)T (rs10902088), MUC2 T(-12150)C (rs10794288) (ЗАО «Синтол»). В проведенном исследовании установлена ассоциация аллеля С (OR = 2,11) и генотипа СС (OR = 2,57) полиморфных вариантов rs833061460 гена VEGFА с повышенным риском развития наружного генитального эндометриоза в славянской популяции Северо-Западного федерального округа.

The aim of the study was to search for the association of the polymorphisms +936C/T and +460C/T of the VEGF gene and polymorphisms of C(-15161)T (rs10902088) and T(-12150)C (rs10794288) of the MUC2 gene with the risk of developing genital endometriosis in the Slavic population of the North-Western Federal District. The study included 60 patients (mean age 32.1 ± 12.4 years) with a diagnosis of external genital endometriosis and 76 healthy donors (mean age 29.6 ± 9.7 years). Genotyping was carried out by real-time PCR using the LightCycler 480 Instrument II (Roche, Switzerland) amplifiers and VEGF T(-460)C (rs833061), VEGF C936T (rs3025039), MUC2 C(-15161)T (rs10902088), MUC2 T(-12150)C (rs10794288) (Sintol). The association of C allele (OR = 2.11) and the СС genotype (OR = 2.57) of polymorphism rs833061460 in VEGFA gene with an increased risk of developing external genital endometriosis in Slavic population of the North-Western Federal District.

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The authors declare that there are no conflicts of interest present.