medgenМедицинская генетикаMedical Genetics2073-7998Publishing House «Genius Media» LLC10.25557/2073-7998.2025.07.104-106medgen-3093Research ArticleКРАТКИЕ СООБЩЕНИЯBRIEF REPORTАнализ ассоциации полиморфизма гена SELP с риском невынашивания беременности в I триместре у женщин Ростовской областиAnalysis of the association between SELP gene polymorphism and the risk of first-trimester pregnancy loss in women from the Rostov regionСуходоловаТ. А.SukhodolovaT. A.

344090, г. Ростов-на-Дону, пр-т Стачки, д. 194/1

194/1 Stachki Avenue, Rostov-on-Don, 344090

МашкинаЕ. В.MashkinaE. V.

344090, г. Ростов-на-Дону, пр-т Стачки, д. 194/1

194/1 Stachki Avenue, Rostov-on-Don, 344090

Южный федеральный университет (ЮФУ), Академия биологии и биотехнологии имени Д. И. ИвановскогоРоссияSouthern federal university (SFedU), D. I. Ivanovsky Academy of Biology and BiotechnologyRussian Federation202529092025247104106Copyright © Суходолова Т.А., Машкина Е.В., 20252025Суходолова Т.А., Машкина Е.В.Sukhodolova T.A., Mashkina E.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.medgen-journal.ru/jour/article/view/3093Введение

Введение. Невынашивание беременности (НБ) остаётся значимой проблемой в современном акушерстве, затрагивая 10–25% всех беременностей. Молекулы клеточной адгезии играют ключевую роль в репродуктивном цикле, и их изменения могут приводить к репродуктивной патологии.

Цель

Цель: изучение ассоциации полиморфизма rs6131 (1057C>T, S290N) гена SELP с риском НБ в I триместре у женщин Ростовской области.

Метод

Метод. В исследовании использовали образцы ДНК, полученные из крови 114 женщин (59 – контрольная группа – женщины с физиологическим течением беременности, 55 – женщины с НБ, в том числе 25 – с самопроизвольным абортом, 30 – с неразвивающейся беременностью). ДНК выделяли термокоагуляционным методом; нуклеотидные замены определяли аллель-специфичной ПЦР.

Результат

Результат. Частота гомозигот по аллелю C составила 66,1% в контрольной группе и 60% в группе с НБ. Распределение частот генотипов и аллелей по rs6131 не различалось между группами женщин (p > 0,05).

Выводы

Выводы. Полиморфизм rs6131 не связан с изменением риска НБ в первом триместре.

Introduction

Introduction. Miscarriage remains a significant problem in modern obstetrics, affecting 10-25% of all pregnancies. Cell adhesion molecules play a key role in the reproductive cycle, and their changes can lead to reproductive pathologies.

Aim

Aim: to study the association of the rs6131 (1057C>T, S290N) polymorphism of the SELP gene with the risk of miscarriage in the first trimester in women of the Rostov region.

Methods

Methods. The study used DNA samples obtained from the blood of 114 women (59 – control group – women with a physiological course of pregnancy, 55 – women with miscarriage (25 – with spontaneous abortion, 30 – with an undeveloped pregnancy)). DNA was isolated by thermocoagulation; nucleotide substitutions were determined by allele-specific PCR.

Results

Results. The frequency of homozygotes for the C allele was 66.1% in the control group and 60% in the miscarriage group. The frequency distribution of genotypes and alleles according to rs6131 did not differ between the groups of women (p > 0.05).

Conclusions

Conclusions. The rs6131 polymorphism is not associated with a change in the risk of miscarriage in the first trimester.

невынашивание беременностиP-селектинполиморфизм генаSELPклеточная адгезияmiscarriageP-selectinpolymorphismSELPcell adhesionРабота была выполнена при финансовой поддержке Министерства науки и высшего образования РФ в рамках государственного задания в сфере научной деятельности № FENW-2023-0018.The work was carried out with the financial support of the Ministry of Science and Higher Education of the Russian Federation within the framework of the state assignment in the field of scientific activity No. FENW-2023-0018.ReferencesШелехин А. П., Баев О. Р., Красный А. М. Роль молекул клеточной адгезии в патогенезе преэклампсии. Акушерство и гинекология. 2021; 6:22-28.Shelekhin A.P., Baev O.R., Krasnyi A.M. Rol’ molekul kletochnoy adgezii v patogeneze preeklampsii [The role of cell adhesion molecules in the pathogenesis of preeclampsia]. Akusherstvo i Ginekologiya [Obstetrics and Gynecology]. 2021; 6: 22-28 (In Russ.)Achache H., Revel A. Endometrial receptivity markers, the journey to successful embryo implantation. Human reproduction update. 2006;12(6):731-746.Achache H., Revel A. Endometrial receptivity markers, the journey to successful embryo implantation. Human reproduction update. 2006;12(6):731-746.Zenclussen A.C., Fest S., Sehmsdorf U.S., et al. Upregulation of decidual P-selectin expression is associated with an increased number of Th1 cell populations in patients suffering from spontaneous abortions. Cell Immunol. 2001;213(2):94-103.Zenclussen A.C., Fest S., Sehmsdorf U.S., et al. Upregulation of decidual P-selectin expression is associated with an increased number of Th1 cell populations in patients suffering from spontaneous abortions. Cell Immunol. 2001;213(2):94-103.Dendana M., Hizem S., Magddoud K., et al. Common polymorphisms in the P-selectin gene in women with recurrent spontaneous abortions. Gene. 2012;495(1):72-75.Dendana M., Hizem S., Magddoud K., et al. Common polymorphisms in the P-selectin gene in women with recurrent spontaneous abortions. Gene. 2012;495(1):72-75.Vlachadis N., Tsamadias V., Siori M., et al. Association of the PECAM-1 (Leu125Val) and P-Selectin (Thr715Pro) Gene Polymorphisms With Unexplained Spontaneous Miscarriages. Cureus. 2022;14(2):e21859.Vlachadis N., Tsamadias V., Siori M., et al. Association of the PECAM-1 (Leu125Val) and P-Selectin (Thr715Pro) Gene Polymorphisms With Unexplained Spontaneous Miscarriages. Cureus. 2022;14(2):e21859.Vlachadis N., Tsamadias V., Vrachnis N., et al. Associations of combined polymorphisms of the platelet membrane glycoproteins Ia and IIIa and the platelet-endothelial cell adhesion molecule-1 and P-Selectin genes with IVF implantation failures. J Obstet Gynaecol. 2017;37(3):363-369.Vlachadis N., Tsamadias V., Vrachnis N., et al. Associations of combined polymorphisms of the platelet membrane glycoproteins Ia and IIIa and the platelet-endothelial cell adhesion molecule-1 and P-Selectin genes with IVF implantation failures. J Obstet Gynaecol. 2017;37(3):363-369.Vlachadis N., Tsamadias V., Kouskouni E., et al. Genetic heterogeneity of platelet glycoproteins Ia and IIIa is associated with in vitro fertilisation implantation failure. J Obstet Gynaecol. 2015;35(7):733-6.Vlachadis N., Tsamadias V., Kouskouni E., et al. Genetic heterogeneity of platelet glycoproteins Ia and IIIa is associated with in vitro fertilisation implantation failure. J Obstet Gynaecol. 2015;35(7):733-6.Patel K. D., Nollert M. U., McEver R. P. P-selectin must extend a sufficient length from the plasma membrane to mediate rolling of neutrophils. The Journal of cell biology. 1995; 131(6). 1893-1902.Patel K. D., Nollert M. U., McEver R. P. P-selectin must extend a sufficient length from the plasma membrane to mediate rolling of neutrophils. The Journal of cell biology. 1995; 131(6). 1893-1902.Ruchaud-Sparagano M.H., Malaud E., Gayet O., et al. Mapping the epitope of a functional P-selectin monoclonal antibody (LYP20) to a short complement-like repeat (SCR 4) domain: use of humanmouse chimaera and homologue-replacement mutagenesis. Biochem J. 1998;332 ( Pt 2)(Pt 2):309-14.Ruchaud-Sparagano M.H., Malaud E., Gayet O., et al. Mapping the epitope of a functional P-selectin monoclonal antibody (LYP20) to a short complement-like repeat (SCR 4) domain: use of humanmouse chimaera and homologue-replacement mutagenesis. Biochem J. 1998;332 ( Pt 2)(Pt 2):309-14.Bugert P., Vosberg M., Entelmann M., et al. Polymorphisms in the P-selectin (CD62P) and P-selectin glycoprotein ligand-1 (PSGL-1) genes and coronary heart disease. Clin Chem Lab Med. 2004;42(9):997-1004.Bugert P., Vosberg M., Entelmann M., et al. Polymorphisms in the P-selectin (CD62P) and P-selectin glycoprotein ligand-1 (PSGL-1) genes and coronary heart disease. Clin Chem Lab Med. 2004;42(9):997-1004.Herrmann S.M., Ricard S., Nicaud V., et al. The P-selectin gene is highly polymorphic: reduced frequency of the Pro715 allele carriers in patients with myocardial infarction. Hum Mol Genet. 1998;7(8):1277-84.Herrmann S.M., Ricard S., Nicaud V., et al. The P-selectin gene is highly polymorphic: reduced frequency of the Pro715 allele carriers in patients with myocardial infarction. Hum Mol Genet. 1998;7(8):1277-84.

The authors declare that there are no conflicts of interest present.