medgenМедицинская генетикаMedical Genetics2073-7998Publishing House «Genius Media» LLC10.1234/XXXX-XXXX-2013-3-20-28medgen-29Research ArticleНАУЧНЫЕ ОБЗОРЫREVIEWНЕОПЛАСТИЧЕСКАЯ ТРАНСФОРМАЦИЯ МУЛЬТИПОТЕНТНЫХ МЕЗЕНХИМАЛЬНЫХ СТРОМАЛЬНЫХ КЛЕТОК В КУЛЬТУРЕ IN VITRONEOPLASTIC TRANSFORMATION OF MULTIPOTENT MESENCHYMAL STROMAL CELLS IN VITROРжаниноваА. А.RzhaninovaA. A.

115478, Москва, ул. Москворечье, 1

115478 Moscow, Moskvorechie st.1

ОмельченкоД. О.OmelchenkoD. O.

115478, Москва, ул. Москворечье, 1

115478 Moscow, Moskvorechie st.1

ФедюнинаИ. А.FeduninaI. A.

115478, Москва, ул. Москворечье, 1

115478 Moscow, Moskvorechie st.1

Федеральное государственное бюджетное учреждение «Медико-генетический научный центр» Российской академии медицинских наукРоссияFederal State Budgetary Institution «Research centre for medical genetics» of the Russian Academy of Med ical Sciences (FSBI «RCMG» RAMS)Russian Federation2013221220151232028Copyright © Ржанинова А.А., Омельченко Д.О., Федюнина И.А., 20152015Ржанинова А.А., Омельченко Д.О., Федюнина И.А.Rzhaninova A.A., Omelchenko D.O., Fedunina I.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.medgen-journal.ru/jour/article/view/29

Ключевую позицию в развитии клеточных технологий занимают исследования мультипотентных мезенхимальных стромальных клеток (ММСК), что обусловлено доступностью их получения, экспансией in vitro и возможностью приготовления трансплантатов из собственных клеток пациента. Внедрение клеточных технологий на основе ММСК в клиническую практику требует решения ряда вопросов, связанных с безопасностью применения клеточного материала в лечебных целях. В первую очередь, это касается риска развития новообразований, обусловленного возможностью спонтанной неопластической трансформации клеток в культуре. При этом помимо клеточного старения как основной причины злокачественной трансформации нельзя также исключить негативное влияние на клетки некоторых манипуляций ex vivo, таких, как генетическая модификация и индукция дифференцировки.

 

Study of multipotent mesenchymal stromal cells (MMSC) hold key position in the development of cell-based technologies due to the availabili ty of their production, expansion in vitro and the possibili ty of preparation of autogenic graft of patient's cells. The introduction of cell-based technologies into clinical practice requires solvi ng a number of issues relating to the safety of cellular material. First it is needed toconsider the risk of tumors caused by spontaneous neoplastic transformation of cells in culture. In this case similarly to celi ular senescence as the main cause of malignant transformation, it is also impossible to exclude a negative effect of some ex vivo manipulations with cells including genetic modification and induction of differentiation.

 

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The authors declare that there are no conflicts of interest present.