medgenМедицинская генетикаMedical Genetics2073-7998Publishing House «Genius Media» LLC10.25557/2073-7998.2024.03.31-37medgen-2454Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL RESEARCHМикросателлитная нестабильность у российских больных с раком желудкаMicrosatellite instability in Russian patients with gastric cancerМолчановА. Д.MolchanovA. D.

119048, г. Москва, ул. Трубецкая, д. 8, стр. 2;

115478, г. Москва, Каширское шоссе, д. 23

8-2, Trubetskaya st., Moscow, 119991;

23, Kashirskoe shosse, Moscow, 115478

m.artem.aug@gmail.comАндриановА. С.AndrianovA. S.

119048, г. Москва, ул. Трубецкая, д. 8, стр. 2

8-2, Trubetskaya st., Moscow, 119991

ВасильеваА. С.VasilyevaA. S.

115478, г. Москва, Каширское шоссе, д. 23

23, Kashirskoe shosse, Moscow, 115478

БуреИ. В.BureI. V.

119048, г. Москва, ул. Трубецкая, д. 8, стр. 2

8-2, Trubetskaya st., Moscow, 119991

КузнецоваЕ. Б.KuznetsovaE. B.

119048, г. Москва, ул. Трубецкая, д. 8, стр. 2;

115522, г. Москва, ул. Москворечье, д. 1

8-2, Trubetskaya st., Moscow, 119991;

1, Moskvorechye st., Moscow, 115522

АлексееваЕ. А.AlekseevaE. A.

119048, г. Москва, ул. Трубецкая, д. 8, стр. 2;

115522, г. Москва, ул. Москворечье, д. 1

8-2, Trubetskaya st., Moscow, 119991;

1, Moskvorechye st., Moscow, 115522

НемцоваМ. В.NemtsovaM. V.

119048, г. Москва, ул. Трубецкая, д. 8, стр. 2;

115522, г. Москва, ул. Москворечье, д. 1

8-2, Trubetskaya st., Moscow, 119991;

1, Moskvorechye st., Moscow, 115522

Первый Московский государственный медицинский университет им. И.М. Сеченова (Сеченовский университет); ФГБУ «Национальный медицинский исследовательский центр онкологии им. Н.Н. Блохина» Минздрава РоссииРоссияI.M. Sechenov First Moscow state medical university; N.N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of the Russian FederationRussian FederationПервый Московский государственный медицинский университет им. И.М. Сеченова (Сеченовский университет)РоссияI.M. Sechenov First Moscow state medical universityRussian FederationФГБУ «Национальный медицинский исследовательский центр онкологии им. Н.Н. Блохина» Минздрава РоссииРоссияN.N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of the Russian FederationRussian FederationПервый Московский государственный медицинский университет им. И.М. Сеченова (Сеченовский университет); ФГБНУ «Медико-генетический научный центр имени академика Н.П. Бочкова»РоссияI.M. Sechenov First Moscow state medical university; Research Centre for Medical GeneticsRussian Federation2024120720242333137Copyright © Молчанов А.Д., Андрианов А.С., Васильева А.С., Буре И.В., Кузнецова Е.Б., Алексеева Е.А., Немцова М.В., 20242024Молчанов А.Д., Андрианов А.С., Васильева А.С., Буре И.В., Кузнецова Е.Б., Алексеева Е.А., Немцова М.В.Molchanov A.D., Andrianov A.S., Vasilyeva A.S., Bure I.V., Kuznetsova E.B., Alekseeva E.A., Nemtsova M.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.medgen-journal.ru/jour/article/view/2454

Введение. Рак желудка (РЖ) является распространенным злокачественным заболеванием, оказывающим значительное влияние на качество жизни пациентов и уровень смертности. Микросателлитная нестабильность (MSI) – это генетическое изменение, которое может влиять на прогрессирование и прогноз рака. В настоящем исследовании изучаются частоты MSI при РЖ у российских пациентов, а также проводится анализ её прогностической значимости.Цель: определить частоту MSI у больных РЖ в России и проанализировать связь с клиническими параметрами, чтобы оценить прогностическую значимость.Методы. Материалом исследования служили 160 парных образцов опухолевой и неопухолевой ткани больных, оперированных по поводу РЖ. Определение MSI проводили путём фрагментного анализа ДНК с использованием пяти мононуклеотидных маркеров (NR21, NR24, NR27, BAT25 и BAT26). Образцы сравнивали с помощью точного критерия Фишера. Для сравнения более 3 групп использовали критерий хи-квадрат. Общая вероятность выживания рассчитывалась методом предельного произведения Каплана-Мейера.Результаты. По результатам исследования MSI была обнаружена у 28 из 160 пациентов, что составляет 17,5%. При анализе связи MSI с клинико–патологическими характеристиками пациентов выявлена достоверная ассоциация с возрастом старше 50 лет (p=0,038), размером опухоли (Т3-Т4) (р=0,017), метастазами в региональные лимфатические узлы (N1-N3) (p=0,028), отдаленными метастазами (М1) (p=0,009) и интестинальным типом по классификации Лорен (p=0,046).

Introduction. Gastric cancer (GC) is a common malignant disease that has a significant impact on patients’ quality of life and mortality rate. Microsatellite instability (MSI) is a genetic change that can influence cancer progression and prognosis. This study examines the frequency of MSI in GC in Russian patients, as well as analyzes its prognostic significance.Aim: to determine the frequency of MSI in patients with gastric cancer in Russia and its relationship with clinical parameters and determine the prognostic significance.Methods. The study material included 160 paired samples of tumor and non-tumor tissue from patients undergoing surgery for gastric cancer. Microsatellite instability was determined by DNA fragment analysis using five mononucleotide markers (NR21, NR24, NR27, BAT25 and BAT26). Samples were compared using Fisher’s exact test. For comparisons of more than three groups, the chi-square test was used. Overall probability of survival (OS) was calculated by the Kaplan-Meier product limit method from the date of surgery to death from any cause and compared statistically using the Mantel-Haenszel test.Results. According to the study results, MSI was found in 28 out of 160 patients, representing 17.5%. When analyzing the associations of MSI with clinical and pathological characteristics of patients, a significant association was revealed with age over 50 years (p=0.038), tumor size (T3-T4) (p=0.017), metastases to regional lymph nodes (N1-N3) (p= 0.028), distant metastases (M1) (p=0.009) and intestinal type according to Lauren’s classification (p=0.046).

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The authors declare that there are no conflicts of interest present.