medgenМедицинская генетикаMedical Genetics2073-7998Publishing House «Genius Media» LLC10.25557/2073-7998.2023.04.32-37medgen-2283Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL RESEARCHПолиморфизм гена ANRIL и предрасположенность к атеросклерозуANRIL gene polymorphism and susceptibility to atherosclerosisТимофееваС. В.TimofeevaS. V.

344090, г. Ростов-на-Дону, пр. Стачки, д. 194/1

194/1,Stachki ave, Rostov-on-Don, 344090

timofeeva.sophia@gmail.comШерчковаТ. А.SherchkovaT. A.

344090, г. Ростов-на-Дону, пр. Стачки, д. 194/1

194/1,Stachki ave, Rostov-on-Don, 344090

ШкуратТ. П.ShkuratT. P.

344090, г. Ростов-на-Дону, пр. Стачки, д. 194/1

194/1,Stachki ave, Rostov-on-Don, 344090

Южный федеральный университет, Академия биологии и биотехнологииРоссияSouthern Federal University, Academy of biology and biotechnologyRussian Federation2023260720232243237Copyright © Тимофеева С.В., Шерчкова Т.А., Шкурат Т.П., 20232023Тимофеева С.В., Шерчкова Т.А., Шкурат Т.П.Timofeeva S.V., Sherchkova T.A., Shkurat T.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.medgen-journal.ru/jour/article/view/2283

Проведен метаанализ для оценки связи между полиморфизмом ANRIL rs2383207 и предрасположенностью к атеросклерозу. Для выявления подходящих исследований был проведен систематический поиск литературы в базах данных Google Scholar и PubMed. Всего для мета анализа было отобрано 12 исследований. Ассоциацию оценивали по статистическому отношению шансов (ОШ) с 95% доверительным интервалом (ДИ). Программное обеспечение RevMan (Cochrane Collaboration, 5.3. Копенгаген) использовали для мета анализа. При объединении исследуемых популяций   в одну выборку показано, что ген ANRIL был связан с риском атеросклероза. Дальнейший стратифицированный анализ по этническому признаку показал, что ген ANRIL был связан с предрасположенностью к атеросклерозу как у азиатов, так и у европеоидов. Наши результаты показывают, что ANRIL может служить генетическим биомаркером атеросклероза. Для подтверждения наблюдаемой связи потребуются дальнейшие исследования.

We conducted this meta-analysis to estimate associations between polymorphisms of ANRIL rs2383207 and susceptibility to atherosclerosis. A systematic literature research of Google Scholar and PubMed was performed to identify eligible studies. Overall, 12 studies were included for meta-analyses. The association was assessed by statistical odds ratio (OR) with 95% confidence interval (CI). RevMan software (Cochrane Collaboration, 5.3. Copenhagen) was used for the meta-analysis. Pooled overall analyses showed that ANRIL was associated with risk of atherosclerosis in the whole population. Further analyses by ethnicity revealed that ANRIL was associated with susceptibility to atherosclerosis in Asians and Caucasions. Our results suggest that ANRIL, might serve as genetic biomarkers of atherosclerosis. Further studies will be required to confirm the observed association.

ANRILднРНКатеросклерозметаанализANRILlncRNAatherosclerosismetaanalysisReferencesDing X.-Q., Ge P.-C., Liu Z., et al. Interaction between microRNA expression and classical risk factors in the risk of coronary heart disease. Scientific Reports. 2015 (9):14925.Ding X.-Q., Ge P.-C., Liu Z., et al. Interaction between microRNA expression and classical risk factors in the risk of coronary heart disease. Scientific Reports. 2015 (9):14925.Ballantyne M.D., McDonald R.A., Baker A.H. lncRNA/MicroRNA interactions in the vasculature. Clin Pharmacol Ther. 2016;99(5):494-501.Ballantyne M.D., McDonald R.A., Baker A.H. lncRNA/MicroRNA interactions in the vasculature. Clin Pharmacol Ther. 2016;99(5):494-501.Jathar S., Kumar V., Srivastava J., Tripathi V. Technological developments in lncRNA biology. Adv Exp Med Biol. 2017;1008:283–323.Jathar S., Kumar V., Srivastava J., Tripathi V. Technological developments in lncRNA biology. Adv Exp Med Biol. 2017;1008:283–323.Archer K., Broskova Z., Bayoumi A.S. et al. Long non-coding RNAs as master regulators in cardiovascular diseases. Int J Mol Sci. 2015;16:23651–23667.Archer K., Broskova Z., Bayoumi A.S. et al. Long non-coding RNAs as master regulators in cardiovascular diseases. Int J Mol Sci. 2015;16:23651–23667.Buysschaert I., Carruthers K.F., Dunbar D.R., Peuteman G., Rietzschel E., Belmans A., Hedley A., Meyer T.D., Budaj A., Van de Werf F., Lambrechts D., Fox K.A.A. A variant at chromosome 9p21 is associated with recurrent myocardial infarction and cardiac death after acute coronary syndrome: The GRACE Genetics Study. Eur. Heart J. 2010;31:1132–1141.Buysschaert I., Carruthers K.F., Dunbar D.R., Peuteman G., Rietzschel E., Belmans A., Hedley A., Meyer T.D., Budaj A., Van de Werf F., Lambrechts D., Fox K.A.A. A variant at chromosome 9p21 is associated with recurrent myocardial infarction and cardiac death after acute coronary syndrome: The GRACE Genetics Study. Eur. Heart J. 2010;31:1132–1141.Timofeeva S.V., Sherchkova T.A., Shkurat T.P. Polymorphism rs2383207 of CDKN2B-AS and Susceptibility to Atherosclerosis: A Mini Review. Non-coding RNA. 2022;8(6):78.Timofeeva S.V., Sherchkova T.A., Shkurat T.P. Polymorphism rs2383207 of CDKN2B-AS and Susceptibility to Atherosclerosis: A Mini Review. Non-coding RNA. 2022;8(6):78.Fasolo F., Di Gregoli K., Maegdefessel L., Johnson J.L. Non-coding RNAs in cardiovascular cell biology and atherosclerosis. Cardiovasc Res. 2019;115(12):1732-1756.Fasolo F., Di Gregoli K., Maegdefessel L., Johnson J.L. Non-coding RNAs in cardiovascular cell biology and atherosclerosis. Cardiovasc Res. 2019;115(12):1732-1756.Congrains A., Kamide K., Oguro R., et al. Genetic variants at the 9p21 locus contribute to atherosclerosis through modulation of ANRIL and CDKN2A/B. Atherosclerosis. 2012;220(2):449-455.Congrains A., Kamide K., Oguro R., et al. Genetic variants at the 9p21 locus contribute to atherosclerosis through modulation of ANRIL and CDKN2A/B. Atherosclerosis. 2012;220(2):449-455.Kong Y., Hsieh C.H., Alonso L.C. ANRIL: A lncRNA at the CDKN2A/B Locus With Roles in Cancer and Metabolic Disease. Front Endocrinol (Lausanne). 2018;9:405.Kong Y., Hsieh C.H., Alonso L.C. ANRIL: A lncRNA at the CDKN2A/B Locus With Roles in Cancer and Metabolic Disease. Front Endocrinol (Lausanne). 2018;9:405.Cunnington M.S., Keavney B. Genetic mechanisms mediating atherosclerosis susceptibility at the chromosome 9p21 locus. Curr Atheroscler Rep. 2011;13(3):193-201.Cunnington M.S., Keavney B. Genetic mechanisms mediating atherosclerosis susceptibility at the chromosome 9p21 locus. Curr Atheroscler Rep. 2011;13(3):193-201.Li J., Chen J., Zhang F., et al. LncRNA CDKN2B-AS1 hinders the proliferation and facilitates apoptosis of ox-LDL-induced vascular smooth muscle cells via the ceRNA network of CDKN2B-AS1/miR-126-5p/PTPN7. Int J Cardiol. 2021;340:79-87.Li J., Chen J., Zhang F., et al. LncRNA CDKN2B-AS1 hinders the proliferation and facilitates apoptosis of ox-LDL-induced vascular smooth muscle cells via the ceRNA network of CDKN2B-AS1/miR-126-5p/PTPN7. Int J Cardiol. 2021;340:79-87.Moher D., Liberati A., Tetzlaff J., Altman D.G.; PRISMA Group. Preferred reporting items for systematic reviews and meta-analyses: the PRISMA statement. Ann Intern Med. 2009;151(4):264-W64.Moher D., Liberati A., Tetzlaff J., Altman D.G.; PRISMA Group. Preferred reporting items for systematic reviews and meta-analyses: the PRISMA statement. Ann Intern Med. 2009;151(4):264-W64.Lo C.K., Mertz D., Loeb M. Newcastle-Ottawa Scale: comparing reviewers’ to authors’ assessments. BMC Med Res Methodol. 2014;14:45.Lo C.K., Mertz D., Loeb M. Newcastle-Ottawa Scale: comparing reviewers’ to authors’ assessments. BMC Med Res Methodol. 2014;14:45.Zhang T., Xu C., Zhao R., Cao Z. Diagnostic Value of sST2 in Cardiovascular Diseases: A Systematic Review and Meta-Analysis. Front Cardiovasc Med. 2021;8:697837.Zhang T., Xu C., Zhao R., Cao Z. Diagnostic Value of sST2 in Cardiovascular Diseases: A Systematic Review and Meta-Analysis. Front Cardiovasc Med. 2021;8:697837.Lin L., Chu H. Quantifying publication bias in meta-analysis. Biometrics. 2018;74(3):785-794.Lin L., Chu H. Quantifying publication bias in meta-analysis. Biometrics. 2018;74(3):785-794.Jin Q., Shi G. Meta-Analysis of SNP-Environment Interaction with Heterogeneity. Hum Hered. 2019;84(3):117-126.Jin Q., Shi G. Meta-Analysis of SNP-Environment Interaction with Heterogeneity. Hum Hered. 2019;84(3):117-126.Abdullah K.G., Li L., Shen G.Q, et al. Four SNPS on chromosome 9p21 confer risk to premature, familial CAD and MI in an American Caucasian population (GeneQuest). Ann Hum Genet. 2008;72(Pt 5):654-657.Abdullah K.G., Li L., Shen G.Q, et al. Four SNPS on chromosome 9p21 confer risk to premature, familial CAD and MI in an American Caucasian population (GeneQuest). Ann Hum Genet. 2008;72(Pt 5):654-657.Çakmak H.A., Bayoğlu B., Durmaz E., et al. Evaluation of association between common genetic variants on chromosome 9p21 and coronary artery disease in Turkish population. Anatol J Cardiol. 2015;15(3):196-203.Çakmak H.A., Bayoğlu B., Durmaz E., et al. Evaluation of association between common genetic variants on chromosome 9p21 and coronary artery disease in Turkish population. Anatol J Cardiol. 2015;15(3):196-203.García-González I., Pérez-Mendoza G., Solís-Cárdenas A., et al. Genetic variants of PON1, GSTM1, GSTT1, and locus 9p21.3, and the risk for premature coronary artery disease in Yucatan, Mexico. Am J Hum Biol. 2022;34(5):e23701.García-González I., Pérez-Mendoza G., Solís-Cárdenas A., et al. Genetic variants of PON1, GSTM1, GSTT1, and locus 9p21.3, and the risk for premature coronary artery disease in Yucatan, Mexico. Am J Hum Biol. 2022;34(5):e23701.Ferreira L.E., Secolin R., Lopes-Cendes I, Cabral N.L., França P.H.C. Association and interaction of genetic variants with occurrence of ischemic stroke among Brazilian patients. Gene. 2019;695:84-91.Ferreira L.E., Secolin R., Lopes-Cendes I, Cabral N.L., França P.H.C. Association and interaction of genetic variants with occurrence of ischemic stroke among Brazilian patients. Gene. 2019;695:84-91.Zhou L., Zhang X., He M., et al. Associations between single nucleotide polymorphisms on chromosome 9p21 and risk of coronary heart disease in Chinese Han population. Arterioscler Thromb Vasc Biol. 2008;28(11):2085-2089.Zhou L., Zhang X., He M., et al. Associations between single nucleotide polymorphisms on chromosome 9p21 and risk of coronary heart disease in Chinese Han population. Arterioscler Thromb Vasc Biol. 2008;28(11):2085-2089.Lin H.F., Tsai P.C., Liao Y.C., et al. Chromosome 9p21 genetic variants are associated with myocardial infarction but not with ischemic stroke in a Taiwanese population. J Investig Med. 2011;59(6):926-930.Lin H.F., Tsai P.C., Liao Y.C., et al. Chromosome 9p21 genetic variants are associated with myocardial infarction but not with ischemic stroke in a Taiwanese population. J Investig Med. 2011;59(6):926-930.Zhang W., Chen Y., Liu .P, et al. Variants on chromosome 9p21.3 correlated with ANRIL expression contribute to stroke risk and recurrence in a large prospective stroke population. Stroke. 2012;43(1):14-21. doi:10.1161/STROKEAHA.111.625442Zhang W., Chen Y., Liu .P, et al. Variants on chromosome 9p21.3 correlated with ANRIL expression contribute to stroke risk and recurrence in a large prospective stroke population. Stroke. 2012;43(1):14-21. doi:10.1161/STROKEAHA.111.625442Wang Q., Zhao J., Chang H., Liu X., Zhu R. Association between lncRNA ANRIL genetic variants with the susceptibility to ischemic stroke: From a case-control study to meta-analysis. Medicine (Baltimore). 2021;100(11):e25113.Wang Q., Zhao J., Chang H., Liu X., Zhu R. Association between lncRNA ANRIL genetic variants with the susceptibility to ischemic stroke: From a case-control study to meta-analysis. Medicine (Baltimore). 2021;100(11):e25113.Chen Z., Qian Q., Ma G., et al. A common variant on chromosome 9p21 affects the risk of early-onset coronary artery disease. Mol Biol Rep. 2009;36(5):889-893.Chen Z., Qian Q., Ma G., et al. A common variant on chromosome 9p21 affects the risk of early-onset coronary artery disease. Mol Biol Rep. 2009;36(5):889-893.Kumar J., Yumnam S., Basu T., et al. Association of polymorphisms in 9p21 region with CAD in North Indian population: replication of SNPs identified through GWAS. Clin Genet. 2011;79(6):588-593.Kumar J., Yumnam S., Basu T., et al. Association of polymorphisms in 9p21 region with CAD in North Indian population: replication of SNPs identified through GWAS. Clin Genet. 2011;79(6):588-593.El-Menyar A.A., Rizk N.M., Al-Qahtani A., et al. The cardiovascular implication of single nucleotide polymorphisms of chromosome 9p21 locus among Arab population. J Res Med Sci. 2015;20(4):346-352.El-Menyar A.A., Rizk N.M., Al-Qahtani A., et al. The cardiovascular implication of single nucleotide polymorphisms of chromosome 9p21 locus among Arab population. J Res Med Sci. 2015;20(4):346-352.Yang J., Gu L., Guo X., et al. LncRNA ANRIL Expression and ANRIL Gene Polymorphisms Contribute to the Risk of Ischemic Stroke in the Chinese Han Population. Cell Mol Neurobiol. 2018;38(6):1253-1269.Yang J., Gu L., Guo X., et al. LncRNA ANRIL Expression and ANRIL Gene Polymorphisms Contribute to the Risk of Ischemic Stroke in the Chinese Han Population. Cell Mol Neurobiol. 2018;38(6):1253-1269.

The authors declare that there are no conflicts of interest present.