medgenМедицинская генетикаMedical Genetics2073-7998Publishing House «Genius Media» LLC10.25557/2073-7998.2021.01.44-56medgen-1841Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL RESEARCHРоль полиморфных вариантов генов ADIPOQ, MTHFR, PON1, KCNJ11, TCF7L2, ITLN1 и PPARG в увеличении риска развития ожирения в Кыргызской РеспубликеThe role and interaction of polymorphic variants of non-allelic genes ADIPOQ, MTHFR, PON1, KCNJ11, TCF7L2, ITLN1, PPARG in the increase in the risk of obesity in Kyrgyz RepublicИсаковаЖ. Т.IsakovaZh. T.jainagul@mail.ruКипеньВ. Н.KipenV. N.noemail@neicon.ruАкынбекКызы СамараAkynbekK. SAMARAnoemail@neicon.ruАйтбаевК. А.AitbaevK. A.noemail@neicon.ruАлдашеваН. М.AldashevaN. M.noemail@neicon.ruТалайбековаЭ. Т.TalaibekovaE. T.noemail@neicon.ruМукееваС. Б.MukeevaS. B.noemail@neicon.ruОсмонкулКызы МээримOsmonkulK. MEERIMnoemail@neicon.ruБейшеналиеваС. Т.BeishenalievaS. T.noemail@neicon.ruМиррахимовЭ. М.MirrakhimovE. M.noemail@neicon.ruНИИ Молекулярной биологии и медициныРоссияInstitute of Molecular Biology and MedicineRussian FederationНИИ Молекулярной биологии и медициныРоссияInstitute of Genetics and Cytology of the National Academy of Sciences of BelarusRussian FederationНациональный центр кардиологии и терапииРоссияNational Center of Cardiology and Internal MedicineRussian Federation2021110320212014456Copyright © Исакова Ж.Т., Кипень В.Н., Акынбек К.С., Айтбаев К.А., Алдашева Н.М., Талайбекова Э.Т., Мукеева С.Б., Осмонкул К.М., Бейшеналиева С.Т., Миррахимов Э.М., 20212021Исакова Ж.Т., Кипень В.Н., Акынбек К.С., Айтбаев К.А., Алдашева Н.М., Талайбекова Э.Т., Мукеева С.Б., Осмонкул К.М., Бейшеналиева С.Т., Миррахимов Э.М.Isakova Z.T., Kipen V.N., Akynbek K.S., Aitbaev K.A., Aldasheva N.M., Talaibekova E.T., Mukeeva S.B., Osmonkul K.M., Beishenalieva S.T., Mirrakhimov E.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.medgen-journal.ru/jour/article/view/1841

Цель исследования - оценить вклад полиморфных вариантов g.15661G> T (ген ADIPOQ), p.A222V (ген MTHFR), p.Q192R (ген PON1), p.K23E (ген KCNJ11), g.53341C>T (ген TCF7L2), p.V109D (ген ITLN1) и p.P12A (ген PPARG) в развитие ожирения для лиц кыргызской национальности. Исследование было проведено по типу «случай-контроль» и включало 130 пациентов с ожирением (мужчин - 65 (50,0%), женщин - 65 (50,0%)). Группа сравнения - 115 человек без ожирения, метаболических нарушений и сердечно-сосудистых заболеваний в анамнезе (мужчин - 62 (53,9%), женщин - 53 (46,1%). Генотипирование осуществляли методом ПЦР-ПДРФ. Анализ межгенных взаимодействий проводился с использованием программы MDR 3.0.2. Маркерами развития ожирения в кыргызской популяции являются аллель V и генотип VV локуса p.A222V (MTHFR), а также аллель K и генотип KK локуса p.K23E (KCNJ11). При наличии генетического профиля AV (p.A222V, MTHFR) / EK (p.K23E, KCNJ11) вероятность развития ожирения возрастает более чем в 3 раза, ОШ=3,49, 95% ДИ=[1,44-8,45], p=0,001.

In this study, we investigated whether polymorphisms g.15661G>T (ADIPOQ), p.A222V (MTHFR), p.Q192R (PON1), p.K23E (KCNJ11), g.53341C>T (TCF7L2), p.V109D (ITLN1) and p.P12A (PPARG) are associated with obesity in the Kyrgyz population. We genotyped 245 nonrelated adults Kyrgyz individuals. 130 patients (male - 65 (50,0%), female - 65 (50,0%) with obesity and 115 non-obese control subjects (male - 62 (53,9%), female - 53 (46,1%). Genotyping of single-nucleotide polymorphism was performed by PCR-RFLP method. Analysis of intergenic interactions conducted with MDR v.3.0.2 program. Тhe V allele and the VV genotype of the p.A222V locus (MTHFR), as well as the K allele and the KK genotype of the p.K23E locus (KCNJ11) increase risk of obesity in the Kyrgyz population. Subjects having the VV genotype of p.A222V locus (MTHFR) and KK genotype of the p.K23E locus (KCNJ11) had 3-fold [OR = 3,49 (1,44-8,45); p = 0,001] higher risk of developing compared with subjects carrying neither of these genotypes.

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The authors declare that there are no conflicts of interest present.