medgenМедицинская генетикаMedical Genetics2073-7998Publishing House «Genius Media» LLC10.25557/2073-7998.2020.11.21-30medgen-1792Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL RESEARCHОментин-1 подкожной жировой ткани при ишемической болезни сердцаSubcutaneous adipose tissue omentin-1 in coronary artery disease patientsПобожеваИ. А.PobozhevaI. A.perhaps_to_be@mail.ruПантелееваА. А.PanteleevaA. A.noemail@neicon.ruПоляковаЕ. А.PolyakovaE. A.noemail@neicon.ruДрачеваК. В.DrachevaK. V.noemail@neicon.ruРазгильдинаН. Д.RazgildinaN. D.noemail@neicon.ruГалкинаО. В.GalkinaO. V.noemail@neicon.ruБеляеваО. Д.BelyaevaO. D.noemail@neicon.ruБерковичО. А.BerkovichO. A.noemail@neicon.ruБарановаЕ. И.BaranovaE. I.noemail@neicon.ruПчелинаС. Н.PchelinaS. N.noemail@neicon.ruМирошниковаВ. В.MiroshnikovaV. V.noemail@neicon.ruПетербургский институт ядерной физики им. Б.П. Константинова, Национальный исследовательский центр «Курчатовский институт»; Первый Санкт-Петербургский государственный медицинский университет имени академика И.П. Павлова Министерства здравоохранения Российской ФедерацииРоссияNational Research Center «Kurchatov Institute»; Acad. I.P. Pavlov First St.-Petersburg State Medical UniversityRussian FederationПервый Санкт-Петербургский государственный медицинский университет имени академика И.П. Павлова Министерства здравоохранения Российской ФедерацииРоссияAcad. I.P. Pavlov First St.-Petersburg State Medical UniversityRussian FederationПетербургский институт ядерной физики им. Б.П. Константинова, Национальный исследовательский центр «Курчатовский институт»РоссияNational Research Center «Kurchatov Institute»Russian Federation20200202202119112130Copyright © Побожева И.А., Пантелеева А.А., Полякова Е.А., Драчева К.В., Разгильдина Н.Д., Галкина О.В., Беляева О.Д., Беркович О.А., Баранова Е.И., Пчелина С.Н., Мирошникова В.В., 20212021Побожева И.А., Пантелеева А.А., Полякова Е.А., Драчева К.В., Разгильдина Н.Д., Галкина О.В., Беляева О.Д., Беркович О.А., Баранова Е.И., Пчелина С.Н., Мирошникова В.В.Pobozheva I.A., Panteleeva A.A., Polyakova E.A., Dracheva K.V., Razgildina N.D., Galkina O.V., Belyaeva O.D., Berkovich O.A., Baranova E.I., Pchelina S.N., Miroshnikova V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.medgen-journal.ru/jour/article/view/1792

Дисбаланс в секреции адипокинов жировой тканью может играть роль в развитии сердечно-сосудистой патологии при ожирении. Оментин-1, один из адипокинов жировой ткани, обладает противовоспалительным, антиоксидантным, антиатерогенным эффектами. Снижение концентрации оментина-1 в сыворотке крови может рассматриваться как биомаркер сердечно-сосудистых заболеваний. Цель работы - исследование экспрессии гена оментина-1 (ITLN1) в подкожной жировой ткани (ПЖТ) у пациентов с ишемической болезнью сердца (ИБС) и в группе сравнения, включающее сравнительный анализ уровня мРНК и белка в ПЖТ, концентрации оментина-1 в сыворотке крови, а также оценку корреляции экспрессии гена ITLN1 и гена ключевого транскрипционного регулятора адипогенеза - рецептора, активируемого пероксисомным пролифератором, гамма (PPARG). Были исследованы образцы ПЖТ и сыворотки крови 74 пациентов с ИБС, перенесших операцию коронарного шунтирования, и 16 пациентов (группа сравнения), оперированных по поводу клапанных пороков сердца. Уровень мРНК генов ITLN1 и PPARG в ПЖТ оценивали методом ПЦР в реальном времени. Уровень белка оментина-1 в ПЖТ определяли методом вестерн-блот. Концентрацию оментина-1 в сыворотке крови измеряли методом ИФА. Концентрация оментина-1 в сыворотке крови была ниже в группе пациентов с ИБС, чем у обследованных из группы сравнения (р<0,01), и отрицательно коррелировала с окружностью талии у всех обследованных (r=-0,307, p<0,01). Не было выявлено различий в содержании мРНК гена ITLN1 и белка оментина-1 в ПЖТ между исследуемыми группами. Уровень мРНК гена ITLN1 коррелировал с уровнем белка оментина-1 в ПЖТ (r=0,373, р<0,05). Более высокий уровень белка оментина-1 в ПЖТ был выявлен у мужчин по сравнению с этим показателем у женщин (р<0,05), однако у женщин концентрация оментина-1 в сыворотке крови была выше, чем у мужчин (р<0,05). Уровень мРНК гена PPARG в ПЖТ был ниже у пациентов с ИБС (р<0,05). Концентрация оментина-1 в сыворотке крови положительно коррелировала с уровнем мРНК гена PPARG в ПЖТ (r=0,338, p<0,05). Среди всех обследованных уровень мРНК гена ITLN1 и уровень белка оментина-1 в ПЖТ отрицательно коррелировали с мРНК гена PPARG в ПЖТ (r=-0,444, p<0,01 и r=-0,475, p<0,01, соответственно). Аналогичные корреляции сохранялись для подгруппы мужчин (r= -0,422, p<0,05 и r= -0,609, p<0,01, соответственно). ИБС, ожирение и мужской пол ассоциированы со снижением концентрации оментина-1 в сыворотке крови. Установлены гендерные особенности регуляции экспрессии гена оментина-1 в ПЖТ.

Imbalance in secretion of adipose tissue adipokines may play a role in the development of cardiovascular pathology associated with obesity. Omentin-1 is an adipokine with antiinflammatory, antioxidant and antiatherogenic properties, therefore it’s serum concentration is considered as a biomarker of cardiovascular diseases. Objective: Investigation of omentin-1 gene (ITLN1) expression in SAT in coronary artery disease (CAD) patients and in the control group, including сomparative analysis of ITLN1 mRNA and protein levels in SAT, omentin-1 serum concentration, as well as an assessment of the correlation of the ITLN1 gene expression with the mRNA level of PPARG gene encoding peroxisome proliferator-activated receptor gamma as the key regulator of adipogenesis. Subcutaneous adipose tissue (SAT) biopsies and serum samples from 74 patients with CAD, undergoing coronary artery bypass grafting, and 16 persons of the control group, were included in the study. ITLN1 and PPARG mRNA levels were detected by quantitative real-time PCR. Omentin-1 protein level in SAT was measured by western-blot. Serum omentin-1 concentration was determined by ELISA. Serum omentin-1 concentration was decreased in the CAD group compared to controls (p<0,01), and inversely correlated with waist circumference among all examined individuals (r = -0.307, p <0.01). No differences were found in the ITLN1 mRNA and omentin-1 protein levels in SAT between the studied groups. The ITLN1 mRNA and omentin-1 protein levels in SAT were positively correlated (r=0,373, p<0.05). A higher level of omentin-1 protein in SAT was detected in men compared with women (p <0.05), however, omentin-1 serum concentration was higher in women (p <0.05). The PPARG mRNA level in SAT was lower in patients with CAD (p <0.05). Omentin-1 serum concentration was positively correlated with the PPARG mRNA level in SAT (r =0.338, p <0.05). The ITLN1 mRNA and omentin-1 protein levels were negatively correlated with the PPARG mRNA in SAT among all examined individuals (r = -0.444, p <0.01 and r = -0.475, p <0.01, respectively). These correlations persisted only in men subgroup when men and women were analyzed separately (r= -0,422, p<0,05 and r= -0,609, p<0,01, respectively). CAD, adiposity and male gender are associated with reduced omentin-1 serum concentration. Gender differences of omentin-1 gene expression regulation in SAT were demonstrated.

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The authors declare that there are no conflicts of interest present.