medgenМедицинская генетикаMedical Genetics2073-7998Publishing House «Genius Media» LLC10.1234/XXXX-XXXX-2016-9-29-39medgen-170Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL RESEARCHГенетические аномалии: влияние на эффективность терапии, выживаемость больных множественной миеломой, роль в оценке минимальной остаточной болезниGenetic anomalies show independent influence on life expectancy of patients at oncological diseasesГарифуллинА. Д.GarifullinA. D.bloodscience@mail.ruВолошинС. В.VoloshinS. V.noemail@neicon.ruМартынкевичИ. С.MartynkevichI. S.noemail@neicon.ruКлеинаЕ. В.KleinaE. V.noemail@neicon.ruСалогубГ. Н.SalogubG. N.noemail@neicon.ruКарягинаЕ. В.KaryaginaE. V.noemail@neicon.ruАбдулкадыровК. М.AbdulkadyrovK. V.noemail@neicon.ruЧечеткинА. В.ChechetkinA. V.noemail@neicon.ruФедеральное государственное бюджетное учреждение «Российский научно-исследовательский институт гематологии и трансфузиологии Федерального медико-биологического агентства»РоссияRussian Research Institute of Hematology and TransfusiologyRussian FederationФедеральное государственное бюджетное образовательное учреждение высшего образования «Первый Санкт-Петербургский государственный медицинский университет им. академика И.П. Павлова» Министерства здравоохранения Российской Федерации; Федеральное государственное бюджетное учреждение «Северо-Западный федеральный медицинский исследовательский центр им. В.А. Алмазова» Министерства здравоохранения Российской ФедерацииРоссияFirst Pavlov State Medical University of St. Peterburg; V.A. Almazov Federal North-West Medical Research Centre of the Ministry of Health of the Russian FederationRussian FederationСанкт-Петербургское государственное бюджетное учреждение здравоохранения Городская больница №15РоссияCity Hospital N15Russian Federation2016131020161592939Copyright © Гарифуллин А.Д., Волошин С.В., Мартынкевич И.С., Клеина Е.В., Салогуб Г.Н., Карягина Е.В., Абдулкадыров К.М., Чечеткин А.В., 20162016Гарифуллин А.Д., Волошин С.В., Мартынкевич И.С., Клеина Е.В., Салогуб Г.Н., Карягина Е.В., Абдулкадыров К.М., Чечеткин А.В.Garifullin A.D., Voloshin S.V., Martynkevich I.S., Kleina E.V., Salogub G.N., Karyagina E.V., Abdulkadyrov K.V., Chechetkin A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.medgen-journal.ru/jour/article/view/170

При ряде онкологических заболеваний генетические аномалии (ГА) демонстрируют самостоятельное влияние на продолжительность жизни больных. В нашем исследовании определена частота встречаемости аномалий генетического аппарата и их прогностический потенциал при множественной миеломе (ММ). Выявление аномалий проводилось стандартным цитогенетическим методом и флуоресцентной гибридизацией in situ (FISH). У пациентов определялись перестройка гена IgH (t(11;14), t(4;14) и др.), del(13)(q14), del(17)(p13.1), аномалии хромосомы 1, гиподиплоидия, гипердиплоидия, сочетанный и комплексный кариотипы. У 57,1% пациентов ГА отсутствовали, а частота встречаемости аномалий хромосомы 1, t(11;14), del(13)(q14), t(4;14), del(17)(p13.1), гипердиплоидии, гиподиплоидии равнялась 28,6%, 20,3%, 18,1%, 6,8%, 5,6%, 3,6 и 2,9% соответственно. Длительность наблюдения составила в среднем 5 лет и показала, что del(17)(p13.1) уменьшала общую выживаемость (ОВ), в то время как t(11;14) не влияла на прогноз. Комплексный кариотип продемонстрировал негативное влияние на показатели выживаемости, в основном, за счет наличия прогностически неблагоприятных ГА (del17p13.1, del13q14, t(4;14), (dup(1q)). Минимальная остаточная болезнь (МОБ) может быть выявлена не только путем многоцветной проточной цитометрии (ПЦ), но и генетическими методами исследований, направленными на выявление ранее обнаруженных ГА.

Genetic anomalies: the influence on efficiency of therapy, survival of patients with multiple myeloma, a role in assessment of minimal residual disease. In our research the frequency of occurrence of genetic anomalies and their predictive potential at multiple myeloma is determined. Detection of anomalies was carried out by standard cytogenetic method and fluorescent in situ hybridization (FISH). IgH reorganization (t(11;14), t(4;14), etc.), del(13)(q14), del(17)(p13.1), anomalies of 1 chromosome, hypodiploidy, hyperdiploidy, combined and complex karyotypes decided at patients with multiple myeloma. Genetic anomalies were detected in 57.1% of patients, the frequency of occurrence of anomalies of 1 chromosome, t(11;14), del(13)(q14), t(4;14), del(17)(p13.1), hyperdiploidy, hypodiploidy - in 28.6%, 20.3%, 18.1%, 6.8%, 5.6%, 3.6 and 2.9%, respectively. Duration of observation has averaged 5 years and has shown that del17p13.1 reduced overall survival, t(11;14) didn’t influence the forecast. The complex karyotype has shown negative impact on survival indicators, generally due to existence predictively of adverse anomalies (del(17)(p13.1), del(13)(q14), t(4;14), (+1q)). The Minimal Residual Disease can be revealed by means of flow cytometry and also genetic methods of researches directed to identification of earlier defined anomalies.

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The authors declare that there are no conflicts of interest present.